摘要
In the present study,we aimed to investigate the chemical constituents from Embelia laeta.The constituents were separated by various column chromatography,including silica gel,ODS gel,MCI gel,Sephadex LH-20,and semipreparative HPLC.Their structures were elucidated by spectroscopic means.A total of 13 compounds were isolated from E.laeta and identified as(6R,9R)-1-Oxo-α-ionol-9-O-β-D-goucopyranoside(1),(+)-Pinoresinol(2),Leptolepisol D(3),Kaempferol(4),Kaempferol-3-α-L-Ramnopyranoside(5),Kaempferol-3-β-D-(6-O-trans-p-coumaroyl) glucopyranoside(6),Bis(2-ethylhexyl) phthalate(7),2-Benzenedicarboxylic diisobutyl ester(8),Caffeic acid methyl ester(9),Paldehyde(10),Coniferaldehyde(11),Caffeic acid(12)and 5-Hydroxymethyl furfural(13),respectively.All the compounds were isolated from E.laeta for the first time.
本研究运用硅胶、C_(18)反相、MCI柱色谱及半制备HPLC等多种色谱分离方法从酸藤子中分离得到13个化合物。通过NMR和MS数据分析,并结合文献数据对比等方法,鉴定了它们的结构,包括(6R,9R)-1-Oxo-α-ionol-9-O-β-Dgoucopyranoside (1),(+)-Pinoresinol (2),Leptolepisol D (3),山柰酚(4),山柰酚-3-α-L-鼠李糖苷(5),Kaempferol-3-β-D-(6-Otrans-p-coumaroyl) glucopyranoside (6),Bis(2-ethylhexyl) phthalate (7),邻苯二甲酸二丁酯(8),咖啡酸甲酯(9),芥子醛(10),Coniferaldehyde (11),咖啡酸(12),5-羟甲基糠醛(13)。所有化合物均为首次从该植物中分离得到。
