MitoQ对犬骨髓间充质干细胞线粒体功能及抗氧化能力的影响

Effects of MitoQ on mitochondrial function and antioxidant capacity of canine bone marrow mesenchymal stem cells

研究线粒体靶向抗氧化剂Mitoquinone(MitoQ)对犬骨髓间充质干细胞(bone marrow mesenchymal stem cells,BMSCs)的线粒体功能与抗氧化基因表达的作用,为进一步提高犬BMSCs的临床应用效率提供理论依据。以犬BMSCs为材料,添加MitoQ预处理48 h,采用荧光定量PCR、荧光探针等方法评估BMSCs线粒体功能以及抗氧化基因的表达水平。结果显示,随着传代次数的增加,BMSCs线粒体融合明显增强,线粒体分裂明显减弱;线粒体膜电位与ATP含量均显著下降;抗氧化基因的表达显著下调。添加MitoQ预处理后,BMSCs线粒体融合基因Mfn2的表达略有上调,且线粒体分裂基因Drp1和线粒体生物合成相关基因PGC-1α的表达显著上调;线粒体膜电位与ATP生成显著增加;抗氧化基因SOD1、SOD2、CAT和GSH-Px的表达显著上调。结果表明,MitoQ可改善犬BMSCs线粒体功能,增强BMSCs抗氧化能力。

The aim of this study was to investigate the effect of Mitoquinone(MitoQ),a mitochondrial-targeted antioxidant,on the mitochondrial function and antioxidant gene expression of canine bone marrow mesenchymal stem cells(BMSCs),which may provide a theoretical basis for improving the clinical application efficiency of canine BMSCs.Canine BMSCs treated with MitoQ for 48 h were used as materials to evaluate the mitochondrial function and the expression level of antioxidant genes.The results showed that with the increase of passage times,the mitochondrial fusion of BMSCs was significantly advanced,and mitochondrial division was significantly weakened.In addition,the mitochondrial membrane potential and ATP content were significantly decreased and the expression of antioxidant genes of BMSCs was also significantly down-regulated.After MitoQ pretreatment,the expression of BMSCs mitochondrial fusion gene Mfn2 was slightly up-regulated,and the expression of mitochondrial division gene Drp1 and mitochondrial biosynthesis-related gene PGC-1αwere significantly up-regulated.More over,the mitochondrial membrane potential and ATP content increased significantly and the expression of antioxidant genes SOD1,SOD2,CAT and GSH-Px was significantly up-regulated.In summary,MitoQ can improve the mitochondrial function and enhance the antioxidant capacity of canine BMSCs.