光学显微成像技术在液-液相分离研究中的应用

Application of Optical Microscopic Imaging Technology in Liquid-Liquid Separation Research

近十年来,科学家们发现液-液相分离在许多生理过程中扮演重要角色,发挥着许多生物学功能。一旦发生异常,将会导致多种疾病产生。在生物学研究中,光学显微成像技术是液-液相分离主要的研究工具之一。本文阐述了几个重要的光学显微成像技术在液-液相分离的研究中的应用和对比,以观察相分离的存在和形成等过程。详细地阐述了运用共聚焦显微技术中的荧光漂白后恢复技术(FRAP)检测其扩散和物质交换的特性,并总结了应用该技术时的成像条件、注意事项和参数应用,为科学研究者提供技术选择的依据。

In the past decade,scientists have found that liquid-liquid separation plays an important role in many physiological processes and plays many biological functions.Its abnormal change will lead to a variety of diseases.In the study of liquid-liquid separation in biology,optical microscopy is the main research tool.This paper describes the applica⁃tion and comparison of several important optical microscopic imaging technologies in the study of liquid-liquid phase sepa⁃ration,to explain the existence and formation process of phase separation,obtain the mobility and diffusion data.The char⁃acteristics of diffusion and material exchange detected by fluorescence recovery after photobleaching(FRAP)in confocal microscopy are described in detail,and the imaging conditions,precautions and parameters are summarized.That might provide the basis for scientific researchers to choose the technology.