右美托咪定对乳腺癌细胞生物学特性及糖类抗原Tn表达的影响

Effects of dexmedetomidine on biological characteristics of and carbohydrate antigen Tn expression in breast cancer cells

目的探讨右美托咪定对乳腺癌细胞生物学特性及糖类抗原Tn表达的影响。方法将乳腺癌MCF-7细胞分为RX组、YMD组、YMZ组、YMG组,分别给予生理盐水、10 nmol/L右美托咪定、50 nmol/L右美托咪定、100 nmol/L右美托咪定干预24 h后,检测细胞增殖抑制率、凋亡率、侵袭能力、迁移能力,以及Tn的mRNA和蛋白的相对表达量。取5例乳腺癌患者的癌组织和癌旁组织,检测Tn的mRNA相对表达量。结果RX组、YMD组、YMZ组、YMG组的细胞增殖抑制率、细胞凋亡率均依次升高,迁移细胞数量、Tn mRNA相对表达量依次降低(均P<0.05)。YMD、YMZ、YMG组侵袭细胞数量、Tn的蛋白相对表达量少于或低于RX组,且YMZ、YMG组侵袭细胞数量、Tn的蛋白相对表达量少于或低于YMD组(均P<0.05)。乳腺癌组织中的Tn mRNA相对表达量高于癌旁组织(P<0.05)。结论右美托咪定或可抑制糖类抗原Tn的表达,从而抑制乳腺癌细胞增殖、侵袭、迁移,并促进其凋亡。

Objective To investigate the effects of dexmedetomidine on biological characteristics of and carbohydrate antigen Tn expression in breast cancer cells.Methods Breast cancer MCF-7 cells were divided into RX group(a 24-hour intervention with normal saline),YMD group(a 24-hour intervention with 10 nmol/L dexmedetomidine),YMZ group(a 24-hour intervention with 50 nmol/L dexmedetomidine)and YMG group(a 24-hour intervention with 100 nmol/L dexmedetomidine).The cells′inhibitory rate for proliferation,the apoptosis rate,the invasion and migration abilities,and the relative expression of Tn mRNA and protein were detected.The cancerous tissues and paracancerous tissues of five patients with breast cancer were harvested to detect the relative expression of Tn mRNA.Results The inhibitory rate of cell proliferation and the apoptosis rate increased and the number of migration cells and the relative expression of Tn mRNA decreased in the sequence of the RX group,the YMD group,the YMZ group and the YMG group(all P<0.05).The number of invasion cells and the relative expression of Tn protein were fewer or lower in the YMD,YMZ and YMG groups than in the RX group,and the number of invasion cells and the relative expression of Tn protein were fewer or lower in the YMZ and YMG groups than in the YMD group(all P<0.05).The relative expression of Tn mRNA in breast cancer tissues was higher than that in paracancerous tissues(P<0.05).Conclusion Dexmedetomidine can inhibit the proliferation,invasion,and migration of breast cancer cells and promote their apoptosis probably by inhibiting the expression of carbohydrate antigen Tn.