刺梨叶提取工艺优化及其抗氧化活性研究

Optimization of Extraction Process and Antioxidant Activity of Active Substance from Rosa Roxburghii Leaves

目的:比较不同极性部位提取物抗氧化能力和成分类型,为刺梨叶所含成分进一步研究提供参考方向。方法:通过正交实验优化刺梨叶供试液提取工艺,将提取液分别依次用乙酸乙酯和水饱和正丁醇依次萃取,得到醇层、乙酸乙酯层、正丁醇层、水层,以DPPH自由基清除率和总抗氧化力为评价指标,比较不同层提取物抗氧化能力。结果:提取条件最佳组合为料液比1∶30、超声时间60 min、乙醇浓度60%。正丁醇层提取物的抗氧化能力最强。结论:刺梨叶中富含多酚黄酮类物质,正丁醇层提取物具有更强的抗氧化能力。

Objective:The antioxidant capacity and component types of extracts from different polar parts were compared to provide a reference direction for the further study of the components contained in Rosa roxburghii leaves.Method:The extraction process of the test solution from Rosa roxburghii leaves was optimized by orthogonal experiment.The extract was extracted with ethyl acetate and water saturated n-butanol in turn to obtain alcohol layer,ethyl acetate layer,n-butanol layer and water layer.The antioxidant capacity of the extracts from different layers was compared with DPPH free radical scavenging rate and total antioxidant capacity.Result:The best combination of extraction conditions was solid-liquid ratio 1∶30,ultrasonic time 60 min and ethanol concentration 60%.N-butanol extract has the strongest antioxidant ability.Conclusion:Rosa roxburghii leaves are rich in polyphenols and flavonoids,and n-butanol layer extract has stronger antioxidant capacity.