基于纳米钴/聚L-半胱氨酸的抗坏血酸电化学传感器的制备与研究

Preparation and study of ascorbic acid electrochemical sensor based on Nano-Co/Poly-L-Cys

本文使用电化学沉积和电化学聚合的方法,将纳米钴(Nano-Co)和L-半胱氨酸(L-Cys)修饰于玻碳电极(GCE)表面,制备了一种简便、高效的抗坏血酸电化学传感器。采用扫描电子显微镜(SEM)、循环伏安法(CV)对修饰电极进行电化学表征。考察了抗坏血酸在该传感器上的电化学行为,并对实验条件进行了优化。在最佳条件下,抗坏血酸在4.04×10^(-6)~5.03×10^(-4)mol·L^(-1)浓度范围内与峰电流值呈良好的线性关系(R^(2)=0.9953),检出限为1.13×10^(-6)mol·L^(-1),加标回收率在96.2%~101.5%之间。该方法可用于对抗坏血酸含量的测定,结果令人满意。

A ascorbic acid(AA)electrochemical sensor based on Nano-Co/Poly-L-Cys was prepared by methods that Nano-Co and Poly-L-Cys were modified on the surface of glassy carbon electrode(GCE)by electrochemical deposition and polymerization.The modified electrodes were characterized by cyclic voltammetry(CV)and scanning electron microscope(SEM).Furthermore,the electrochemical behaviors of AA on the electrochemical sensor and the optimal experimental conditions were studied.Under selected conditions,the oxidation peak currents and concentrations of AA have a good linear relationship in the range from 4.04×10^(-6) to 5.03×10^(-4)mol·L^(-1)(R^(2)=0.9953).And there was a low detection limit of 1.13×10~6 mol·L^(-1).When the sensor was used for the analysis of AA in pills,and the recoveries with satisfactory results were in the range of 96.2%and 101.5%.The method was applied to the determination of ascorbic acid with satisfactory results.