牛血清白蛋白修饰电色谱整体柱的微波辅助合成研究

Microwave assisted synthesis and evaluation of monolithic column modified by bovine serum albumin for capillary electrochromatography

借助微波辅助合成技术制备有机聚合物整体柱,对反应体系中各组分的配比进行优化,并采用扫描电子显微镜和红外光谱对聚合效果进行表征。然后利用氨基和环氧基之间的开环反应,将牛血清白蛋白修饰于整体柱表面,并将其应用于异丙肾上腺素的手性分离。实验结果表明,在一定的电泳条件下对异丙肾上腺素实现了基线分离,分离度达到7.73。

The organic polymer monolithic column was prepared by microwave-assisted synthesis technology,the ratio of each component in the reaction system was optimized,and the polymerization effect was characterized by Scanning electron microscope and Infrared spectrum.Then,bovine serum albumin(BSA)was modified on the surface of monolithic column by the ring opening reaction between amino group and epoxy group.The results showed that the baseline separation of isoproterenol was achieved under certain electrophoresis conditions,and the resolution reached 7.73.