黄花软紫草的多糖成分及其抗病毒活性研究

Studies on Polysaccharide from Arnebia Guttata Bunge and Its Antiviral Activity

采用水提醇沉法,提取黄花软紫草水溶性多糖,利用紫外-可见分光光度法、傅立叶变换红外光谱法和1-苯基-3-甲基-5-吡唑啉酮柱前衍生高效液相色谱(PMP-HPLC)法对其化学成分进行了分析.应用半叶枯斑法分析了黄花软紫草水溶性多糖抗烟草花叶病毒(TMV)活性.通过半叶枯斑法分析了其抗烟草花叶病毒(TMV)活性.结果表明,黄花软紫草水溶性多糖AG90的糖含量和蛋白质含量分别为81.0%和8.1%.红外光谱结果呈现明显的多糖特征吸收峰,结构为吡喃环.AG90是由6种单糖构成的水溶性杂多糖,组成单糖如下:甘露糖、鼠李糖、葡萄糖、半乳糖、木糖和阿拉伯糖.抗TMV结果显示,当AG90的浓度为500 mg/mL时,其抗TMV的保护、治疗和钝化活性均优于阳性对照药利巴韦林.

The water-soluble polysaccharide of Arnebia guttata Bunge was extracted by water extraction and alcohol precipitation.Its chemical components were analyzed by UV-vis spectrophotometry,Fourier transform infrared spectroscopy and 1-phenyl-3-methyl-5-pyrazolone Precolumn Derivatization High Performance Liquid Chromatography(PMP-HPLC).Its activity against tobacco mosaic virus(TMV)was analyzed by half leaf blight method.The results showed that the sugar content and protein content of water-soluble polysaccharide AG90 were 81.0%and 8.1%respectively.The results of FT-IR spectra showed that AG90 had obvious polysaccharide characteristic absorption peak,and its structure was pyran ring.AG90 was composed of mannose,rhamnose,glucose,galactose,xylose and arabinose and it was proved to be a water-soluble polysaccharide.AG90 was a water-soluble heteropolysaccharide composed of mannose rhamnose,glucose,galactose,xylose and arabinose.The anti-TMV results showed that when the concentration of AG90 was 500 mg/mL,its anti-TMV protective,therapeutic and passivation activities were better than those of ribavirin.