小鼠视神经损伤后视网膜中TLR-9与MyD88表达变化

Expression changes of TLR-9 and MyD88 in retina of mice following optic nerve injury

目的:探讨Toll样受体-9(TLR-9)与髓样分化因子(MyD88)在小鼠视神经损伤(ONI)后视网膜中的表达变化。方法:选取8周大小的雄性C57BL/6J小鼠36只,随机分为6组:空白对照组(未做任何处理)、ONI 1d组(视神经损伤后1d取材)、ONI 3d组(视神经损伤后3d取材)、ONI 5d组(视神经损伤后5d取材)、ONI 7d组(视神经损伤后7d取材)、ONI 14d组(视神经损伤后14d取材)。分组后通过视神经夹持的方法制作小鼠视神经损伤模型,利用RT-qPCR与Western-blot检测各组小鼠视网膜中TLR-9与MyD88的mRNA和蛋白水平。结果:ONI 1d组视网膜中TLR-9与MyD88的mRNA和蛋白水平与空白对照组比较无差异(P>0.05);ONI 3d、ONI 5d、ONI 7d、ONI 14d组视网膜中TLR-9与MyD88的mRNA和蛋白水平与空白对照组比较均明显增加(P<0.01)。与空白对照组比较视网膜中TLR-9与MyD88的mRNA和蛋白水平在小鼠ONI 3d开始升高(P<0.01),ONI 5d达到峰值(P<0.001),ONI 7d开始逐渐下降(P<0.01)。结论:小鼠视神经损伤能够激活视网膜中TLR-9与MyD88表达,TLR-9与MyD88可能在视神经损伤的进程中起重要作用。

AIM:To investigate the expression changes of Toll like recepter 9(TLR-9)and myeloid differentiation factor 88(MyD88)in retina of mice following optic nerve injury(ONI).METHODS:There were 36 male 8-week-old C57BL/6J mice randomly divided into 6 groups:blank control(no treatment),ONI 1d group(materials were taken at 1d after optic nerve injury),ONI 3d group(materials were taken at 3d after optic nerve injury),ONI 5d group(materials were taken at 5d after optic nerve injury),ONI 7d group(materials were taken at 7d after optic nerve injury),ONI 14d group(materials were taken at 14d after optic nerve injury).The mice optic nerve model was made by optic nerve gripping,and the mRNA and protein levels of Toll like recepter 9 and myeloid differentiation factor 88 in each retinal were measured by RT-qPCR and Western-blot.RESULTS:The mRNA and protein levels of Toll like recepter 9 and myeloid differentiation factor 88 in the retina of ONI 1d group were not significantly different from those of the blank control group(P>0.05),the mRNA and protein levels of TLR-9 and MyD88 in the retina of ONI 3d group,ONI 5d group,ONI 7d group and ONI 14d group were significantly increased compared with the blank control group,and the differences were statistically significant(P<0.01).Compared with the blank control group,the mRNA and protein levels of TLR-9 and MyD88 in the retina of mice began to increase at ONI 3d(P<0.01),peaked at ONI 5d(P<0.001),and gradually decreased at ONI 7d(P<0.01).CONCLUSION:Optic nerve injury can activate the expression of TLR-9 and MyD88 in mice retina.TLR-9 and MyD88 may play an essential role in the process of optic nerve injury.