甜菜TIFY基因家族的全基因组鉴定与生物信息学分析

TIFY Gene Family in Sugar Beet:Whole Genome Identification and Bioinformatics Analysis

TIFY转录因子对植物体生长发育和胁迫响应有重要调控作用,本研究目的在于鉴定分析甜菜(Beta vulgaris L.)中的TIFY转录因子。试验以甜菜基因组数据为基础,利用生物信息学技术在全基因组水平鉴定并分析甜菜TIFY家族成员。结果表明:甜菜中共有21条TIFY基因,基因间序列相似性较低;共线性分析发现只有BvTIFY15与BvTIFY18之间存在基因复制事件;BvTIFY转录因子家族包括2个TIFY蛋白、8个JAZ蛋白、6个ZML蛋白、5个PPD蛋白;蛋白互作预测发现,JAZ家族成员对JA信号有重要的调控作用。本研究对BvTIFY基因的结构与功能进行分析,发掘出甜菜根中应答盐胁迫基因BvTIFY13与BvTIFY15,为后续Bv TIFY基因的功能研究提供一定理论基础。

TIFY transcription factors play an important role in regulating plant growth and development as well as stress response,therefore,the purpose of this study is to identify and analyze TIFY transcription factors in sugar beet(Beta vulgaris L.).Based on the genome data of sugar beet,bioinformatics techniques were used to identify and analyze the members of sugar beet TIFY family at the whole genome level.The results showed that there were 21 TIFY genes in sugar beet with low sequence similarity.Collinearity analysis showed that only BvTIFY15 and BvTIFY18 had gene replication events.The family of BvTIFY transcription factors included 2TIFY proteins,8 JAZ proteins,6 ZML proteins and 5 PPD proteins.Protein-protein interaction prediction showed that members of the JAZ family played an important role in the regulation of JA signaling.This study analyzed the structure and function of BvTIFY genes and discovered the salt stress responsive genes BvTIFY13and BvTIFY15 in sugar beet roots.It can provide a theoretical basis for subsequent functional studies on BvTIFY genes.