梨果仙人掌果多酚分布及生物活性

Distribution and bioactivity of polyphenols in Opuntia ficus-indica(L.)Mill

目的了解梨果仙人掌果多酚分布,并研究其抗氧化和抑制糖消化酶活性。方法以乙醇为提取溶剂,分离出梨果仙人掌果果皮、果肉和种子中的游离多酚.、酯化多酚和不可溶键合多酚,Folin-Ciocalteu法测定多酚含量,通过DPPH自由.基、羟自由基、ABTS^(+)·清除实验测定其抗氧化能力,观察各部位各种类型多酚对α-葡萄糖苷酶和α-淀粉酶活性的体外抑制作用。结果梨果仙人掌果中果皮多酚含量为(1427.15±34.48)mg GAE/100g,果肉多酚含量为(525.88±51.66)mg GAE/100 g,种子多酚含量为(256.92±3.81)mg GAE/100g;梨果仙人掌果不同部位的游离多酚、酯化多酚、不可溶键合多酚对DPPH自由基、羟自由基、ABTS^(+)·均有一定的清除能力,各部位间以种子的抗氧化能力最强;梨果仙人掌果果皮、果肉和种子的酯化多酚、不可溶键合多酚对α-葡萄糖苷酶和α-淀粉酶活性均有抑制作用,以种子多酚抑制能力最强。结论梨果仙人掌果果皮多酚含量最高,种子多酚体外抗氧化和抑制糖消化酶作用最强。

OBJECTIVE To study the distribution of the polyphenols of Opuntia ficus-indica(L.) Mill, and study its antioxidant and the inhibition of sugar digestive enzyme activities by vitro experiments.METHODS Using ethanol as solvent, free polyphenols, esterified polyphenols, and insoluble bonded polyphenols were extracted from the peel, pulp and seeds of Opuntia ficus-indica(L.) Mill, and their contents were determined by the Folin-Ciocalteu method. Antioxidant activity was measured according to their ability to scavenge DPPH, OH and ABTS;radicals, and the inhibition of α-glucosidase and α-amylase activities by various types of polyphenols in various parts was observed in vitro.RESULTS The peel polyphenol content of Opuntia ficus-indica(L.) Mill was(1427.15±34.48) mg GAE/100 g, the pulp polyphenol content was(525.88±51.66) mg GAE/100 g, and the seed polyphenol content was(256.92±3.81) mg GAE/100 g. The free polyphenols, esterified polyphenols, and insoluble bonded polyphenols of different parts of Opuntia ficus-indica(L.) Mill have the ability to scavenge DPPH, OH and ABTS;radicals, and the seeds had the strongest antioxidant capacity among all parts, the esterified polyphenols and insoluble bonded polyphenols of pulp and seeds of Opuntia ficus-indica(L.) Mill have inhibitory effects on the activities of α-glucosidase and α-amylase, with seed polyphenols have the strongest inhibitory ability.CONCLUSION Opuntia ficus-indica(L.) Mill has the highest content of polyphenols in the peel, and seed polyphenols have good antioxidant and the inhibition of sugar digestive enzyme activities.