摘要
为研究添加复合乳酸菌和葡萄籽单宁对紫花苜蓿(Medicago sativa L.)青贮品质及小肠可吸收氨基酸的影响,选用凋萎后二茬现蕾期紫花苜蓿为材料,设置对照(CK),葡萄籽单宁(G)、复合乳酸菌(戊糖乳杆菌+戊糖片球菌+乳酸片球菌,L)和复合乳酸菌+葡萄籽单宁(LG)组,青贮60 d后测定其品质,并采用体外三步法测定其小肠可吸收氨基酸含量。结果表明:与CK组相比,G和LG组青贮饲料乳酸、乙酸、丁酸、总挥发性脂肪酸和氨态氮含量均显著降低(P<0.05);L和G组粗蛋白含量显著提高(P<0.05)。与其他三组相比,G组青贮饲料的Ile,Leu,Lys,His,Arg,Tyr和总氨基酸含量显著提高,菌体蛋白以及小肠可吸收Ile,Lys,His,Arg,Ser和总氨基酸含量显著提高(P<0.05)。因此,添加葡萄籽单宁对紫花苜蓿青贮品质有一定改善作用,明显提高青贮饲料中Lys,His和Arg以及总氨基酸的小肠可吸收量。
This study was aimed to study the effects of compound lactic acid bacteria and grape seed tannins on the fermentation quality and the small intestinal absorbable amino acids of alfalfa(Medicago sativa L.)silage.Regrowth of alfalfa was harvested at the budding stage and ensiled without(control,CK)or with grape seed tannin(G),compound lactic acid bacteria(Lactobacillus pentosus+Pediococcus pentosaceus+Pediococcus lactis,L),and compound lactic acid bacteria+grape seed tannins(LG)after wilting.Silage fermentation characteristics and the small intestinal absorbable amino acids(in vitro)were determined after 60 days of ensiling.The results showed that:compared with the control group,the G and LG group had lower(P<0.05)contents of lactic acid,acetic acid,total volatile fatty acid,and ammonia nitrogen,and the L and G group had higher(P<0.05)content of crude protein.Compared with the other groups,the G group had higher(P<0.05)content of Ile,Leu,Lys,His,Arg,Tyr,and total amino acid in silage,the yield of the rumen microbial protein,and contents of the small intestine absorbs Ile,Lys,His,Arg,Ser,and total amino acid.Therefore,the addition of grape seed tannins had a limited effect on improving the fermentation quality of alfalfa silage,but it increased the small intestine absorbable contents of essential amino acid Lys,His,Arg,and the total amino acid in silage.
作者
张海铉
霍文婕
陈雷
刘强
张拴林
王聪
许庆方
郭刚
ZHANG Hai-xuan;HUO Wen-jie;CHEN Lei;LIU Qiang;ZHANG Shuan-lin;WANG Cong;XU Qing-fang;GUO Gang(College of Animal Science, Shanxi Agricultural University, Taigu, Shanxi Province 030801, China)
出处
《草地学报》
CAS
CSCD
北大核心
2022年第5期1302-1309,共8页
Acta Agrestia Sinica
基金
国家自然科学基金(32001405)资助。
关键词
紫花苜蓿
青贮饲料
乳酸菌
葡萄籽单宁
小肠可吸收氨基酸
Alfalfa
Silage
Lactic acid bacteria
Grape seed tannins
Small intestine absorbable amino acids