德国洋甘菊精油纳米胶囊的制备及抗真菌性能研究

Study on Preparation and Antifungal Properties of Matricaria chamomilla Essential Oil Nanocapsules

[目的]为拓宽德国洋甘菊精油在抑菌方面的应用,以德国洋甘菊精油为芯材,壳聚糖为壁材制备德国洋甘菊精油纳米胶囊。[方法]采用离子凝胶法制备德国洋甘菊精油纳米胶囊,并对所制备的德国洋甘菊精油纳米胶囊进行包埋率测定;同时采用菌丝生长速率法测定德国洋甘菊精油纳米胶囊对水稻纹枯病菌、小麦赤霉病菌、玉米弯孢病菌的抗菌性能。[结果]德国洋甘菊精油纳米胶囊的包埋率为36.33%,抑菌试验表明德国洋甘菊精油纳米胶囊对水稻纹枯病菌、小麦赤霉病菌、玉米弯孢病菌均有抑制作用,EC_(50)值分别为4.61、15.51、12.20 mg/mL。[结论]德国洋甘菊精油纳米胶囊对植物病原真菌有一定的抑制作用,具有开发为天然杀菌剂的潜力。

[Objective]In order to broaden the application of Matricaria chamomilla essential oil in antibacterial,Matricaria chamomilla essential oil nanocapsules were prepared with Matricaria chamomilla essential oil as the core material and chitosan as the wall material.[Method]The Matricaria chamomilla essential oil nanocapsules were prepared by ionic gelation method,and the encapsulation rate of the prepared Matricaria chamomilla essential oil nanocapsules was measured.At the same time,the mycelial growth rate method was used to determine the antibacterial properties of Matricaria chamomilla essential oil nanocapsules against Rhizoctonia solani,Fusarium graminearum and Curvularia lunata.[Result]The encapsulation rate of Matricaria chamomilla essential oil nanocapsules was 36.33%,and the inhibition experiments showed that Matricaria chamomilla essential oil had the inhibitory effect on Rhizoctonia solani,Fusarium graminearum and Curvularia lunata with the EC_(50) values of 4.61,15.51 and 12.20 mg/mL,respectively.[Conclusion]Matricaria chamomilla essential oil nanocapsules have a certain inhibitory effect on phytopathogenic fungi and have the potential to be developed as natural fungicides.