平欧榛子分离蛋白提取条件优化

Optimization of Extraction Conditions of Flat-European Hybrid Hazelnut Protein Isolated

本文采用碱溶酸沉法提取平欧榛子分离蛋白,依托单因素试验分别考察了料液比、pH值、提取时间、温度等因素对榛子分离蛋白提取率的影响,优化分离蛋白提取条件。结果表明,最佳提取条件为pH值9.0,温度50 ℃,提取时间60 min,料液比1∶15(g∶mL),在此条件下平欧榛子分离蛋白提取率为73.64%。

The extraction and separetion technology of alkali extraction and acid precipitation was adopted to prepare protein isolated from hazelnut in this research.The single factor experiment was used to investigate the influence of factors such as ratio of solid to liquid,pH value,extraction time and temperature on the extraction rate of hazelnut protein isolate,and the conditions of protein extraction were optimized.The results showed that the optimum extraction conditions were pH value 9.0,temperature 50 ℃,extraction time 60 min,material liquid ratio 1∶15 (g∶mL).In this condition,the extraction rate of protein isolated of hazelnut can reach 73.64%.