钛表面载银的硫酸乙酰肝素/壳聚糖抗菌涂层的构建

Construction of heparin sulfate/chitosan antibacterial polyelectrolyte membrane loaded with silver on titanium surface

目的研究多孔钛表面通过层层自组装技术(LBL)制备硫酸乙酰肝素(HEP)/壳聚糖(CHI)聚电解质多层膜并沉积纳米银后的抗菌性能。方法应用LBL将带负电的HEP和带正电荷的CHI交替吸附在多孔钛表面形成聚电解质多层膜,在碱性条件下装载银离子。使用扫描电子显微镜、X射线光电子能谱仪、接触角测量仪、电感耦合等离子体发射光谱仪评价各制备表面性质,利用抑菌圈法、浊度法、活/死细菌染色法和涂布平板法评估载银聚电解质多层膜的抗菌性能。结果材料表征手段证实聚电解质多层膜成功沉积在钛表面,且纳米银颗粒沉积在膜表面。抗菌实验显示载银修饰显著改善涂层抗菌能力。结论通过层层自组装技术在改性钛表面制备的载银HEP-CHI聚电解质多层膜具有良好的抗菌性能。

Objective To study the antibacterial properties of nano-silver-containing polyelectrolyte multilayer films assembled with heparan sulfate(HEP)and chitosan(CHI)by layer-by-layer self-assembly technology(LBL)on porous titanium surface.Methods Negatively charged HEP and positively charged CHI were alternately adsorbed by LBL to form polyelectrolyte multilayer films,which were deposited on the surface of porous titanium,and then silver ions were loaded on the multilayer films under alkaline conditions.The surface was evaluated by scanning electron microscopy,X-ray photoelectron spectrometer,a measuring instrument for contact angle and inductively coupled plasma emission spectrometer.The antibacterial effect was evaluated by inhibition zone,turbidity method,live/dead bacterial staining and coated plate method.Results Material characterization methods showed polyelectrolyte multilayer films were successfully deposited on the surface of titanium,and nano-silver particles were loaded.Antibacterial experiments showed that the silver-loaded modification significantly improved the antibacterial ability of the coating.Conclusion Silver-loaded HEP-CHI polyelectrolyte multilayer films are deposited on the surface of modified titanium by LBL,which can inhibit the adhesion of bacteria.