基于表里关联和多元统计分析的黄连药材分级研究

Grading of Rhizoma Coptidis Based on Appearance Trait and Intrinsic Quality Correlation and Multivariate Statistical Analysis

目的:探讨外观性状与内在质量相关联的黄连药材量等级评价方法和标准。方法:以市场收集的18批黄连药材为研究对象,构建外观性状与内在质量相关联的黄连的质量等级评价手段。对18批黄连药材进行了水分、灰分、浸出物、长度、直径、小檗碱含量、表小檗碱含量、黄连碱含量、巴马汀含量和色度检测;运用主成分分析、相关性分析、方差分析等多种数理统计手段,筛选分级指标,进行黄连等级划分研究。结果:经筛选认为长度、浸出物和小檗碱含量为黄连品质评价的关键指标,且外在指标(长度)与内在质量(浸出物和小檗碱含量)在0.01水平上呈显著性正相关,结合市场实际情况,制定了黄连一等、二等、统货的质量等级标准。结论:该质量等级不仅从外观特性上对黄连药材进行了等级评价,同时也在其内在质量差异上进行了表征,补充了现有团体标准的不足,也为后续行业标准或国家标准的建立提供依据。

Objective:To investigate the evaluation method and standard of the quantity rating of Huanglian medicinal materials related to the external traits and intrinsic quality.Methods:To construct a method for evaluating the quality grades of Rhizoma Coptidis based on correlation between appearance traits and intrinsic quality by using 18 batches of commercially available Rhizoma Coptidis.Eighteen batches of Rhizoma Coptidis were tested for moisture,ash,extractum,length,diameter,berberine content,epiberberine content,coptisine content,palmatine content and chromaticity.Mathematical and statistical tools such as principal component analysis(PCA),correlation analysis and analysis of variance were used to screen the grading indexes.Results:Length,extractum and berberine content were considered as the key indexes for the quality evaluation of Rhizoma Coptidis,and the extrinsic index(length)and intrinsic quality indexes(extractum and berberine content)were positively correlated at 0.01 significance level.Finally,the standards of first grade,second grade and general grade quality of Rhizoma Coptidis were developed in combination with the actual market situation.Conclusion:The quality grades not only evaluated Rhizoma Coptidis in terms of appearance characteristics,but also characterized it in terms of intrinsic quality differences,which made up deficiencies for the existing group standards and also provided the basis for establishing subsequent industry standards or national standards.