蝉花抑菌抗氧化谱效关系的初步研究

Preliminary Study on Relationship between Antimicrobial and Antioxidant Spectra of Isaria cicadae Miquel

研究了人工蝉花不同部位之间的抑菌、抗氧化关系并筛选其有效成分。建立人工蝉花不同部位的超高效液相色谱(UPLC)指纹图谱,并对蝉花孢子粉和孢子梗部位的提取物进行体外抑菌和1,1-二苯基-2-三硝基苯肼(DPPH)抗氧化试验。采用聚类分析和灰色关联度法将药效学数据与指纹图谱共有峰进行关联,建立谱效关系。蝉花不同部位提取物均具有较好的抑制金黄色葡萄球菌和清除自由基的作用,其中蝉花孢子粉提取物的治疗效果更好。灰色关联分析表明,蝉花的抑菌和抗氧化作用是各成分共同作用的结果,特征峰为峰23、峰2、鸟苷和腺苷(关联度>0.85),对药效贡献较大。通过建立人工蝉花不同部位间的初步谱效学关系,筛选并鉴定了一些蝉花中对药效贡献较大的特征峰,为进一步研究蝉花的药效成分及药理作用提供参考。

This study aimed to establish the bacteriostasis and antioxidant relationship among different parts of artificially cultivated Isaria cicadae Miquel(IcM)and screen the active components.Ultra-performance liquid chromatography(UPLC)fingerprints of different parts of IcM were established,and in vitro antibacterial and DPPH antioxidant activity were performed on the extracts of spore powder and sterigmata.The pharmacodynamic data was correlated with the common peaks of chromatographic fingerprints by the cluster analysis and grey relational analysis to establish the spectrum-activity relationship.The extracts from different parts of IcM were effective in inhibiting Staphyloccocus aureus and scavenging free radicals.The extract from IcM spore powder showed better therapeutic effect than that form sterigmata.Grey relational analysis showed that the bacteriostasis and antioxidant activities of IcM were the combined effects of various components,and the characteristic peaks were No.23,No.2,guanosine and adenosine(correlation degree>0.85),which greatly contributed to the pharmacodynamics.The preliminary spectrum-activity relationship of different parts of IcM was established in this study,and the characteristic peaks which greatly contributed to the pharmacodynamics of IcM were screened out,providing reference for the further study of the pharmacodynamics and pharmacology of IcM.