金黄色葡萄球菌污染牛奶后的代谢产物研究

Study on Metabolites of Polluted Milk by Staphylococcus aureus

为解决食品安全中存在的隐患,作者构建了气相色谱-质谱(GC-MS)结合顶空固相微萃取(HS/SPME)技术用于探究金黄色葡萄球菌的挥发性代谢产物,并结合代谢组学和基因组学分析牛奶中金黄色葡萄球菌的差异性代谢产物及其代谢途径。结果表明,金黄色葡萄球菌的差异性代谢物包括N-甲硫基甲酰胺、1-丁醇、乙酸、乙苯、均三甲苯、辛酸、苯甲酸、正癸酸、十二烷酸、乙醇、苯酚和丙二醇。之后对其进行基因组学分析得到金黄色葡萄球菌主要是以基础的生命代谢为主,明确了代谢网络中参与代谢的物质(乙醇、乙酸、1-丁醇)的代谢途径和相关基因。本研究为金黄色葡萄球菌的生物标志物研究提供了理论基础。

In order to solve the hidden danger of food safety,gas chromatography-mass spectrometry(GC-MS)combined with headspace solid phase microextraction(HS/SPME)was constructed to explore the volatile metabolites of Staphylococcus aureus,and the different metabolites and metabolic pathways of Staphylococcus aureus in milk were analyzed by the combination of metabonomics and genomics.The results showed that the different metabolites of Staphylococcus aureus included N-methylthioformamide,1-butanol,acetic acid,ethylbenzene,mesitylene,octanoic acid,benzoic acid,n-decanoic acid,dodecanoic acid,ethanol,phenol and propylene glycol.After genomic analysis,Staphylococcus aureus was mainly based on the basic life metabolism,and the metabolic pathways and related genes of the substances involved in metabolism(ethanol,acetic acid,1-butanol)in the metabolic network were identified.This study could provide a theoretical basis for the study of biomarkers of Staphylococcus aureus.