鸡蛋、鸡肉及奶粉中22种有机氯农药残留的测定

Determination of 22 Organochlorine Pesticides in Egg,Chicken and Milk Powder

建立了在鸡蛋、鸡肉和奶粉中同时测定22种有机氯农药残留的方法。样品经乙腈提取、Captiva EMR-Lipid固相萃取柱净化、正己烷复溶后,用气相色谱仪检测,外标法定量。在0.005~0.2 mg/L范围内,22种有机氯农药的质量浓度与其对应的峰面积之间线性关系良好,R^(2)均大于0.995。在0.01、0.02、0.1 mg/kg 3个添加水平下,重复测定6次,22种有机氯农药的回收率均在60%~110%,RSD均小于10%,符合GB 27404—2008的实验室内变异系数要求。该方法能够有效去除鸡蛋、鸡肉和奶粉中的动物脂肪及亲脂性干扰物,方法灵敏度、准确度和精密度均符合农药残留检测的要求,为有效监测鸡蛋、鸡肉和奶粉中有机氯农药残留量提供了一种高效、可靠的分析手段。

A method for simultaneous determination of 22 organochlorine pesticides residues in eggs,chicken and milk powder was established.The samples were extracted by acetonitrile solution,purified by Captiva EMR-Lipid solid phase extraction column,determined by gas chromatography(ECD)after n-hexane dissolution,and quantified with external standard method.The results showed that the linear relationship between the mass concentration of 22 organochlorine pesticides and their corresponding peak areas were good in the range of 0.005~0.2 mg/L,and the R^(2) were greater than 0.995.At spiked levels of 0.01,0.02,0.1 mg/kg,the recoveries of 22 organochlorine pesticides were between 60%~100%with RSD less than 10%after 6 repeated determinations,fitting the coefficient of variation in laboratory according to GB 27404-2008.This method could effectively remove animal fat and lipophilic interference in eggs,chicken and milk powder.Besides,the sensitivity,accuracy and precision of this method could meet the requirements of pesticides residue detection,providing an efficient and reliable analytical method for monitoring organochlorine pesticide residues in eggs,chicken and milk powder.