透明剂对生物组织光学相干层析成像的影响研究

Effect of Optical Clearing Agents on Optical Coherence Tomography of Biological Tissue

生物组织光透明技术针对生物组织具有的高散射及浑浊特性,严重影响生物组织成像的对比度及分辨率等问题,提供了较好的解决办法。把离体猪皮表面涂抹不同浓度的聚乙二醇、甘油、丙二醇这些光学透明剂(Optical Clearing Agents,OCA),利用光学相干断层析(Optical Coherence Tomography,OCT)技术对其进行动态监测,有效地提取测量信号在渗透过程中随测量深度的变化。结果表明,利用光透明技术均可增加OCT的成像深度,但对于不同OCA不同深度的作用效果不同。该研究为了解OCA浓度及作用时间对于不同深度最优选择,提供有价值的数据参考,进一步理解了OCA的作用机制,对于OCA在医学方面的应用有一定的指导作用。

Tissue optical clearing provides a good solution to some problems,such as biological tissue has high scattering and turbidity,which seriously affects the contrast and resolution of biological tissue imaging.Ex vivo porcine skin surfaces are coated with OCA such as polyethylene glycol,glycerol and propanediol in different concentrations.Use OCT to dynamically monitor it,and effectively extract the measured signal as it changes with the measured depth during penetration.The results show that the optical transparency technology can increase the imaging depth of OCT.However,for different OCA,the effect of different depth is different.This study provides a valuable data reference for the optimal choice of OCA concentration and time for different depths.This study gives us a further understanding of the mechanism of OCA and will be helpful in guiding the use of OCA in medicine.