河南省温县铁棍山药根腐线虫种类鉴定

Identification of the nematode pathogen causing root lesion nematode disease in Dioscorea polystachya in Wenxian,Henan province

为明确河南省温县铁棍山药病原线虫种类,在温县铁棍山药种植区采集有明显症状的块茎进行线虫分离,观察线虫的雌、雄成虫的形态特征,并扩增rDNA的28S D2-D3序列对其进行分子生物学鉴定。结果表明,从山药块茎中分离出的根腐线虫形态学特征及测量指标与咖啡短体线虫Pratylenchuscoffeae基本一致,其28S D2-D3序列与P.coffeae相似性达99.87%~100%。本研究明确了河南省温县地区铁棍山药根腐线虫为咖啡短体线虫,为山药线虫病的鉴别及防控提供了科学依据。

In order to clarify the nematode pathogen species of Dioscorea polystachya root lesion nematode rot disease in Wenxian of Henan province,the tubers with obvious symptoms were collected for isolating nematodes from the planting region of yam in November,2020.The pathogenic nematodes were identified by observing the traditional morphological characteristics of female and male adults,and molecular biological identification was carried out based on 28S D2-D3 sequence of rDNA.The results showed that the morphological characteristics and measurement indexes of root lesion nematodes isolated from yam tubers were consistent with those of Pratylenchus coffeae,and the 28S D2-D3 sequence was 99.87%-100%identical to that of P.coffeae.This study confirmed for the first time that the root lesion nematode of D.polystachya in Wenxian of Henan province was P.coffeae,and provided a scientific basis for the identification and control of yam nematode diseases.