鮟鱇鱼、鱿鱼、马面鱼明胶提取工艺优化

Optimization of Lophiiformes,squid,Thamnaconus septentrionalis fish skin gelatin extraction process

为得到最优的鱼皮明胶提取工艺,通过响应面法对鮟鱇鱼、鱿鱼、马面鱼鱼皮进行提胶处理,优化了酶添加量、酶解时间、酶解温度,以及提胶时间和提胶温度。结果显示:鮟鱇鱼皮明胶提取最优工艺,酶添加量0.6%、酶解时间2 h,酶解温度40℃、提胶时间1 h、提胶温度75℃;鱿鱼皮明胶提取最优工艺,酶添加量0.6%、酶解时间2 h,酶解温度40℃、提胶时间1 h、提胶温度75℃;马面鱼皮明胶提取最优工艺,酶添加量0.6%、酶解时间1 h,酶解温度30℃、提胶时间2 h、提胶温度65℃。与传统酸法、碱法等方法所得明胶得率11.23%相比,采用响应面工艺优化的鮟鱇鱼皮明胶提高至24.598%,鱿鱼皮明胶得率从6.82%增长到20.807%,马面鱼皮明胶得率为11.227%。研究表明,本研究的响应面工艺优化方法能有效提高明胶得率,为水产品明胶提取技术提供参考。

In order to obtain the optimal extraction process of fish skin gelatin,the gelatinization treatment of Lophiiformes,squid,and Thamnaconus septentrionalis fish skin was carried out by the response method,and the amount of enzyme addition,enzymolysis time,enzymolysis temperature,as well as the time and amount of gelatin extraction were optimized.The results showed that the optimal extraction process for gelatin from anglerfish skin:enzyme addition 0.6%,enzymolysis time 2 h,enzymolysis temperature 40℃,gel extraction time 1h,gel extraction temperature 75℃;The optimal extraction technology of squid skin gelatin:enzyme addition amount 0.6%,enzymolysis time 2 h,enzymolysis temperature 40℃,gelatin extraction time 1h,gelatin extraction temperature 75℃;The optimal extraction technology of horse noodle fish skin gelatin:enzyme addition amount 0.6%,enzymolysis time 1h,enzymolysis temperature 30℃,gelatin extraction time 2 h,gelatin extraction temperature 65℃.Compared with the traditional acid method,alkali method and other methods,the yield of gelatin was 11.23%.The yield of anglerfish skin gelatin optimized by the response surface process was increased to 24.598%,and the yield of squid skin gelatin increased from 6.82%to 20.807%.The extraction rate of horse noodle fish skin gelatin was 11.227%.Studies have shown that the response surface process optimization method in this study can effectively improve the yield of gelatin and provide a reference for the extraction technology of aquatic products gelatin.