四种血酮检测仪对奶牛酮病诊断效果的比较分析

Comparative Analysis of the Diagnostic Effect of Four Blood KetoneMeters on Dairy Cow Ketosis

目的:比较四种血酮检测仪和生化分析仪的测试奶牛血液中β-羟丁酸的浓度结果,分析血酮检测仪对奶牛酮病临床诊断的效果,为奶牛场对血酮检测仪的使用选择提供依据。方法:本试验选取41头2~5胎次、产后5~25天的泌乳奶牛经尾静脉采血进行β-羟丁酸浓度的测试。应用四种血酮检测仪(桂林优利特、美国雅培、北京怡成和台湾暐世)与生化分析仪对比检测奶牛血液中β-羟丁酸含量的相关性和准确度。结果:四种血酮检测仪与生化分析仪相关性高,线性回归分析结果均>0.95;当测试结果绝对偏差≤±0.2或相对偏差≤±20%时,四种血酮检测仪的合格率均达100%;四种血酮仪测试的均值与生化分析测试结果进行配对T检验,结果显示差异均无统计学意义(P>0.05)。结论:四种血酮检测仪的临床测试性能与生化分析仪接近,均满足用于奶牛血酮检测的要求,可用于奶牛酮病的筛查,其中桂林优利特品牌的血酮仪性价比相对较高,更适合奶牛场用于奶牛酮病的快速筛查。

Objective:To compare the results of testing the content ofβ-hydroxybutyric acid in the blood of dairy cows with four blood ketone meters and biochemical meters,this paper analyzed the effect of blood ketone meters on the clinical diagnosis of ketosis in dairy cows,and provided a basis for the selection of blood ketone meters for dairy farms.Methods:Fourty-one dairy cows with 2~5 parities and 5~25 days postpartum were collected through the tail vein for blood.Four blood ketone meters(Guilin Urit,American Abbott,Beijing Yicheng and Taiwan Visteon)were used to compare with biochemical meters to detectβ-hydroxybutyric acid in dairy cows blood.Results:The four blood ketone detectors have high correlation with the biochemical analyzer,and the linear regression analysis results>0.95;when the absolute deviation of the test result was≤±0.2 or the relative deviation was≤±20%,the pass rate of the four blood ketone detectors all reach 100%.The P values of the four blood ketone meter tests and the biochemical analysis test results were subjected to a paired T test,and the results showed that there was no statistical significant(P>0.05).Conclusion:The clinical test performance of the four blood ketone detectors was close to that of the biochemical analyzer,and they all met the requirements for the detection of blood ketones in dairy cows,and can be used for the screening of ketosis in dairy cows.Among them,the blood ketone meter of Guilin Urit was relatively cost-effective.It was more suitable for dairy farms to quickly screen for ketosis in dairy cows.