CO_(2)浓度对真姬菇后熟阶段生物学参数的影响

Effects of CO_(2) Concentration on Biological Parameters of Post-maturing Stage of Hypsizygus marmoreus

以真姬菇后熟期菌包(60~120 d)为试材,设定低CO_(2)浓度(0.05%~0.20%)、中CO_(2)浓度(0.35%~0.50%)、高CO_(2)浓度(0.70%~1.00%)3个不同CO_(2)浓度处理,研究了不同CO_(2)浓度培养下菌包上、中、下3个部分的理化性能及胞外酶活性,拟得出最适CO_(2)的浓度范围,以期为工厂化生产真姬菇提供参考依据。结果表明:在中、低CO_(2)浓度范围,菌包上、中、下3个部分的pH、可溶性蛋白质含量、总糖含量以及羧甲基纤维素酶、木聚糖酶、漆酶、锰过氧化物酶、淀粉酶等活性变化趋势大体一致,中CO_(2)浓度培养时,120 d时上、中、下部指标一致性较好,说明菌包成熟度高;而在同等条件下用高CO_(2)浓度培养的菌丝代谢已出现抗逆性紊乱。综上所述,CO_(2)浓度对菌包产量有影响,中等CO_(2)浓度的菌包出菇产量最高(520.40 g·袋^(-1)),而CO_(2)浓度过低(496.66 g·袋^(-1))或过高(453.33 g·袋^(-1))均不利于提高菌包产量。

Taking the bacterial bag of Hypsizygus marmoreus in the ripening stage(60-120 days) at test material, three treatments with different CO_(2) concentrations, low(0.05%-0.20%)、medium(0.35%-0.50%) and high(0.70%-1.00%) level of CO_(2) concentrations were set, the effects of the CO_(2) concentration to the upper、middle and lower parts of the package yield were studied, the optimum concentration range of CO_(2) was drawn up, in order to provide reference for industrialized production of Hypsizygus marmoreus.The results showed that several characters including the pH,the soluble protein and total sugar contents, and the activities of carboxymethyl cellulase, xylanase, laccase, manganese peroxidase, amylase had the same trend, when the concentrations of CO_(2) were controlled in a medium or low level.When the packet cultivated 120 days under medium concentrations, indicators from the upper, middle and lower parts of package were most consistent than other concentrations.It indicated that maturity of the pack was well.However, under the condition with high concentrations of CO_(2),the hyphae metabolism had appeared as anti-reverse disorder.Furthermore, the concentration of CO_(2) had an effect on the yield.The results indicated those incubated with a medium level of CO_(2) concentration producted the highest yield(520.40 g·bag^(-1)),and other samples performed smaller package yields, no matter under a low(496.66 g·bag^(-1)) or high(453.33 g·bag^(-1)) level of CO_(2) concentrations.