基于液体颗粒计数器的铜离子快速检测方法研究

A method of rapidly detecting Cu^(2+) based on liquid particle counter

针对水质中重金属检测方法存在操作复杂、检测周期长和检测成本高等问题,采用电阻法研制了液体颗粒计数器并结合点击化学法构建了重金属铜离子检测方法。通过建立颗粒粒径识别模型和颗粒计数模型,开发基于LabVIEW的颗粒计数软件,将聚苯乙烯微球作为检测探针、颗粒计数器作为检测平台,实现了水中铜离子的快速检测。结果显示,颗粒粒径识别相对误差小于5%,颗粒计数相对误差小于10%,说明颗粒计数准确度满足要求。铜离子浓度在10~1000μmol/L时,微球数量与铜离子浓度具有良好的线性相关性,平均回收率在87.85%~101.08%,检测限为2.29μmol/L,检测时间约4 min。结果表明,相比常规的铜离子检测方法,基于电阻法液体颗粒计数器的铜离子检测方法具有操作简单、检测速度快、灵敏度高、检测成本低等优势。

A liquid particle counter was developed with electrical resistance method and a method of detecting heavy metal Cu^(2+) combined with the click chemistry method to solve the problems including the complicated operation,long cycle of detection and high cost of detection existed in the current methods of detecting heavy metals in water quality.A model for identifying the particle size and a model for particle counting were established.A particle counting software based on LabVIEW was developed.A rapid method of detecting Cu^(2+) in water was realized by using polystyrene microspheres as detection probes and particle counters as detection platforms.The results showed that the relative error of particle size identification and particle counting was less than 5% and 10%,indicating that the accuracy of particle counting meets the requirements.When the concentration of Cu^(2+) was 10-1000μmol/L,there was a good linear correlation between the number of polystyrene microspheres and the concentration of Cu^(2+).The average recovery rate was between 87.85%and 101.08%.The detection limit was 2.29μmol/L.The time of detection was about 4 min.Compared with the traditional method of detecting Cu^(2+),the method of detecting Cu^(2+) with a liquid particle counter based on the electrical resistance method has the advantages of simple operation,fast speed of detection,high sensitivity and low cost of detection.