褪黑素促进糖氧剥夺诱导的BV2小胶质细胞向M2极化

Melatonin promoted M2 polarization of BV2 microglia induced by glucose and oxygen deprivation

目的:研究褪黑素处理对糖氧剥夺(OGD)诱导的小鼠小胶质细胞系BV2细胞功能的影响。方法:BV2细胞分为对照组(control)、氧糖剥夺组(OGD)和褪黑素处理组(OGD+Mel)。利用流式细胞仪检测各组细胞CD16/32和兔多克隆抗体CD206的表达,real time RT-PCR方法检测BV2细胞中iNOS和Arg-1 mRNA表达,利用酶联免疫吸附试验(ELISA)方法检测BV2细胞培养上清中IL-1β和IL-4的水平。结果:OGD诱导可促进BV2细胞向M1极化,表现为CD16/32上调、iNOS mRNA表达增加,培养上清中IL-1β水平升高;经过褪黑素处理后,CD16/32、iNOS mRNA,培养上清中IL-1β表达下降,CD206、Arg-1和IL-4表达增加。结论:OGD可诱导BV2细胞向M1方向分化,而褪黑素可使OGD诱导的BV2细胞向M2极化。

Objective:To study the effect of melatonin treatment on the function of mouse microglial cell line BV2 induced by glucose and oxygen deprivation(OGD).Methods:BV2 cells were divided into control group(control),oxygen and sugar deprivation group(OGD)and melatonin treatment group(OGD+Mel).Flow cytometry was used to detect the expression of CD16/32 and rabbit polyclonal antibody CD206.Real-time RT-PCR was used to detect the mRNA expression of iNOS and Arg-1 in BV2 cells.The levels of IL-1βand IL-4 in the supernatant of BV2 cell culture were determined by enzyme-linked immunosorbent assay(ELISA).Results:OGD could promote the polarization of BV2 cells towards M1,which showed that CD16/32 was up-regulated,iNOS mRNA expression was increased,and IL-1βlevel was increased in supernatant.After melatonin treatment,CD16/32,iNOS mRNA,IL-1βexpression in culture supernatant decreased,CD206,Arg-1,and IL-4 expression increased.Conclusion:Melatonin can promote M2 polarization of BV2 microglia induced by glucose and oxygen deprivation.