摘要
目的:制备载阿霉素超声纳米泡(Dox-NBs),评价其基本特性并对比Dox-NBs联合诊断超声(DUS)与超声治疗仪(TUS)作用于鼻咽癌细胞的体外实验研究。方法:通过薄膜水化法、机械振荡法制备Dox-NBs并检测其基本物理特性、载药量及包封率;体外药物释放实验分为Dox-NBs组、Dox-NBs+DUS组、Dox-NBs+TUS组3组,利用多功能酶标仪检测Dox累积释放量;培养鼻咽癌5-8F细胞,按照不同的处理方法分为4组:对照组、Dox-NBs组、Dox-NBs+DUS组、Dox-NBs+TUS组,各组处理3 h后完全培养基培养3 d,细胞计数试剂盒(CCK-8)法检测各组细胞活性。结果:Dox-NBs的平均粒径为(286.93±14.45)nm,Zeta电位(-13.3±2.17)mV,平均载药量(37.0±3.34)μg/mg,包封率(36.30±1.92)%;体外药物释放结果显示,Dox-NBs+DUS组与Dox-NBs+TUS组分别在2 h与24 h Dox的累积释放量比较,差异均有统计学意义(P<0.05);CCK-8检测细胞活性显示,Dox-NBs组的细胞存活率高于Dox-NBs+DUS组与Dox-NBs+TUS组(P<0.01),Dox-NBs+DUS组的细胞存活率最低,Dox-NBs+DUS组对鼻咽癌细胞活性抑制作用最大。结论:Dox-NBs联合辐照可以提高药物释放量及协同抑制鼻咽癌细胞活性,DUS联合Dox-NBs作用效果优于TUS联合Dox-NBs,DUS可作为更普及、简便工具介导载药纳米泡的药物释放。
Objective:An in vitro experimental study that aims to prepare doxorubicin-loaded ultrasound nanobubbles(Dox-NBs),evaluate their basic properties and compare Dox-NBs combined with diagnostic ultrasound(DUS)and therapeutic ultrasound(TUS)acting on nasopharyngeal carcinoma cells.Methods:Dox-NBs was prepared by film hydration and mechanical oscillation and their basic physical properties,drug loading and encapsulation rate were detected.The in vitro drug release experiments involved three groups:Dox-NBs group,Dox-NBs+DUS group and Dox-NBs+TUS group.The cumulative release of Dox was detected by multimode microplate reader;the nasopharyngeal carcinoma 5-8F cells were cultured and divided into 4 groups according to different treatment methods:control group,Dox-NBs group,Dox-NBs+DUS group,Dox-NBs+TUS group,and each group was treated with complete medium for 3 h and then cultured in complete medium for 3 days.The cell counting kit-8(CCK-8)was used to detect the cell activity of each group.Results:The mean particle size of Dox-NBs was(286.93±14.45)nm,zeta potential(-13.3±2.17)mV,mean drug loading(37.0±3.34)μg/mg,and encapsulation rate(36.30±1.92)%.The in vitro drug release results showed that the differences were statistically significant(P<0.05)when comparing the cumulative release of Dox in Dox-NBs+DUS group and Dox-NBs+TUS group at 2 h and 24 h,respectively.CCK-8 assay of cell activity showed that the cell survival rate of Dox-NBs group was higher than that of Dox-NBs+DUS group and Dox-NBs+TUS group(P<0.01).The cell survival rate of Dox-NBs+DUS group was the lowest,and the Dox-NBs+DUS group had the greatest inhibitory effect on nasopharyngeal carcinoma cell activity.Conclusion:Combined irradiation of doxorubicin-loaded nanobubbles with ultrasound can improve drug release and synergistically inhibit nasopharyngeal carcinoma cell activity.The effect of DUS combined with Dox-NBs is superior to that of TUS combined with Dox-NBs,and diagnostic ultrasound can be used as a more accessible and easier tool to mediate drug release from drug-laden nanobubbles.
作者
陆柳桂
黄钊希
韦丽艳
高泳
Lu Liugui;Huang Zhaoxi;Wei Liyan;Gao Yong(Department of Ultrasound,The First Affiliated Hospital of Guangxi Medical University,Nanning 530021,China)
出处
《广西医科大学学报》
CAS
2022年第5期792-797,共6页
Journal of Guangxi Medical University
基金
广西自然科学基金面上项目资助(No.2018GXNSFAA281171)。
关键词
诊断超声
超声治疗仪
载阿霉素超声纳米泡
鼻咽癌细胞
阿霉素
diagnostic ultrasound
ultrasonic therapeutic apparatus
doxorubicin-loaded ultrasound nanobubbles
nasopharyngeal carcinoma cells
doxorubicin