武夷菌素高产基因工程菌株Streptomyces albulus OoWysR活性成分的分离鉴定

Isolation and identification of active ingredients from high wuyiencin-producing genetically engineered strain Streptomyces albulus OoWysR

【背景】武夷菌素高产基因工程菌株Streptomyce albulus OoWysR具有明显的抑菌效果。【目的】明确S.albulus OoWysR的活性成分。【方法】采用柱层析法,利用大孔吸附树脂、离子交换树脂和高效液相色谱等对S.albulus OoWysR的活性成分进行分离纯化,经高分辨率电喷雾电离质谱(high-resolution electrospray ionization mass spectrometry,HR-ESI-MS)和核磁共振(nuclear magnetic resonance,NMR)等波谱技术对化合物化学结构进行鉴定,并通过生长速率法测定化合物的生物活性。【结果】从S.albulus OoWysR中分离鉴定出4个化合物,分别为对羟基苯甲酸(1)、吡咯-2-羧酸(2)、对羟基苯乙醇(3)和云南霉素(4)。化合物1对玉米弯孢病菌、番茄叶霉病菌、玉米小斑病菌和烟草赤星病菌具有一定的抑制作用;化合物2对番茄灰霉病菌、大豆菌核病菌、苹果腐烂病菌、玉米小斑病菌、小麦赤霉病菌、稻瘟病菌和烟草赤星病菌具有一定的抑制作用;化合物3对番茄灰霉病菌、苹果轮纹病菌、玉米小斑病菌和黄瓜炭疽病菌具有一定的抑制作用。【结论】S.albulus OoWysR的活性成分除武夷菌素外还包括对羟基苯甲酸、吡咯-2-羧酸、对羟基苯乙醇和云南霉素。本研究结果为进一步开发与利用该菌株提供了理论依据。

[Background]High wuyiencin-producing genetically engineered strain Streptomyces albulus OoWysR showcases pronounced antifungal effect.[Objective]To identify the active ingredients of S.albulus OoWysR.[Methods]The active ingredients of S.albulus OoWysR were separated and purified by the column chromatography of macroporous resin,ion-exchange resin column chromatography,and high-performance liquid chromatography,respectively.The chemical structures of compounds were identified by high-resolution electrospray ionization mass spectrometry(HR-ESI-MS),nuclear magnetic resonance(NMR),and other spectral methods.The biological activity of compounds was determined with the growth rate method.[Results]Four compounds were isolated and identified from S.albulus OoWysR,which were respectively(1)p-hydroxybenzoic acid,(2)pyrrole-2-carboxylic acid,(3)p-hydroxyphenethyl alcohol,and(4)yunnanmycin.Compound 1 showed inhibitory effect on Curvularia lunata,Fulvia fulva,Alternaria alternata,and Bipolaris maydis.Compound 2 exerted certain inhibitory activity against Botrytis cinerea,Sclerotinia sclerotiorum,B.maydis,Valsa ceratosperma,Fusarium graminearum,Pyricularia oryzae,and A.alternata.Compound 3 had specific inhibitory activity against B.cinerea,B.maydis,Botryosphaeria dothidea,and Colletotrichum gloeosporioides.[Conclusion]The active ingredients of S.albulus OoWysR include p-hydroxybenzoic acid,pyrrole-2-carboxylic acid,p-hydroxyphenethyl alcohol,and yunnanmycin,in addition to wuyiencin.This study provides a theoretical basis for further development and utilization of this strain.