摘要
为明确昆诺阿藜链格孢叶斑病病原,在山西省昆诺阿藜种植区采集典型症状的标本分离病原菌,选择代表性菌株LGB-b和LGB-h对其形态学、分子生物学、致病性及生物学特性进行了研究。综合形态学和多基因系统发育(Alt a 1、endoPG和OPA10-2)分析,确定昆诺阿藜链格孢叶斑病病原为Alternaria alternata。致病性测定发现接种6 d后病斑呈灰绿至黄绿色,表面具有灰棕至灰褐色霉层,周围具黄绿色晕圈,与田间症状基本一致。菌株LGB-b和LGB-h均可侵染昆诺阿藜、藜和台湾藜。菌株LGB-b菌丝生长的最适培养基为V8蔬菜汁琼脂培养基(V8)、温度为25-30℃、水活度≥0.98、pH为6-7;分生孢子萌发的最适水活度≥0.98、pH为6-7。菌株LGB-h菌丝生长的最适培养基为马铃薯胡萝卜琼脂培养基(PCA)、温度为20-25℃、水活度≥0.98、pH为6-7;分生孢子萌发的最适水活度≥0.98、pH为7-8。
Specimens with typical Alternaria leaf spot symptoms on Chenopodium quinoa were collected in the cultivation area of quinoa in Shanxi Province.The representative isolates LGB-b and LGB-h were tested for verifying pathogenicity and the classification status by using molecular characteristics(Alt a 1,endoPG,and OPA10-2)combined with morphological identification.The results indicated that the quinoa Alternaria leaf spot is caused by Alternaria alternata.Pathogenicity test results showed that the leaves showed typical symptoms in 6 days after inoculation,which matched the symptoms in field,manifesting gray-green spot,grey-brown mould,and yellow-green halo.Isolates LGB-b and LGB-h could infect C.quinoa,C.album,and C.formosanum.The influence of culture medium,temperature,water activity(aw),and pH on the growth of representative isolates was studied.It was found that the most favorable medium for growth of mycelia of isolate LGB-b was V8,and the most suitable temperatures were 25-30°C.The aw higher than 0.98 and pH 6-7 were optimal for both mycelia growth and conidial germination of isolate LGB-b.For growth of mycelia of isolate LGB-h,the most favorable medium was PCA,the most suitable temperatures were 20-25°C,the optimum aw was higher than 0.98,and the optimum pH was 6-7.The aw higher than 0.98 and pH 7-8 were optimal for conidial germination of isolate LGB-h.
作者
陈亚蕾
田淼
孙江伟
周建波
任璐
秦楠
殷辉
赵晓军
CHEN Yalei;TIAN Miao;SUN Jiangwei;ZHOU Jianbo;REN Lu;QIN Nan;YIN Hui;ZHAO Xiaojun(College of Plant Protection,Shanxi Agricultural University,Taiyuan 030031,Shanxi,China)
出处
《菌物学报》
CAS
CSCD
北大核心
2022年第5期713-729,共17页
Mycosystema
基金
山西省重点研发项目(201903D221014)
山西省基础研究计划资助项目(20210302123419)
省部共建有机旱作农业国家重点实验室课题(202105D121008-3-3)。
关键词
藜
台湾藜
叶部病害
形态学
致病性
Chenopodium album
Chenopodium formosanum
leaf disease
morphology
pathogenicity