低级别胶质瘤SLC12A5的表达及临床意义的生信分析

Clinical meanings of SLC12A5 expression in low-grade glioma based on analysis of bioinformatics methods

目的探讨溶质载体家族12成员5(SLC12A5)在低级别胶质瘤(LGG)中的表达及临床意义。方法利用GEPIA数据库分析SLC12A5在LGG的表达以及SLC12A5表达与LGG病人预后的关系;通过GEMEMANIA和SDTRING数据库构建SLC12A5的相互作用网络;筛选50个与SLC12A54存在强相关关系的基因并进行功能富集分析。结果SLC12A5在LGG组织中表达显著下调,Kaplan-Meier生存曲线显示SLC12A5低表达与LGG病人不良生存预后有关。相互作用网络结果显示SLC12A5可能与STK39存在相互作用关系。功能富集分析发现,SLC12A5及其共表达基因主要参与化学突触传递、突触小泡周期、认知、细胞连接组织、突触小泡回收以及体液水平的调节等生物学过程。结论SLC12A5在LGG中呈低表达,与病人不良预后相关。

Objective To analyze the expression of solute carrier family 12 member 5(SLC12A5)in low-grade glioma(LGG)based on bioinformatics.Methods The expression of SLC12A5 in LGG and the relationship between expression of SLC12A5 and prognosis of LGG patients were analyzed by GEPIA database.The interaction network of SLC12A54 was constructed by GEMEMANIA and SDTRING databases.Functional enrichment analysis of 50 genes with strong correlation with SLC12A54 was perdormed by Metascape databases.Results SLC12A5 was significantly downregulated in LGG tissues.Kaplan-Meier survival curves showed that low expression of SLC12A5 was associated with poorer survival of LGG patients.Interaction network results indicated a possible interaction between SLC12A5 and STK39.Functional enrichment analysis revealed that SLC12A5 and its co-expressed genes are mainly involved in biological processes such as chemical synaptic transmission,synaptic vesicle cycle,cognition,cellular junctional organization,synaptic vesicle recycling,and regulation at the somatic level.Conclusions SLC12A5 is lowly expressed in LGG tissues and correlated with poor clinical prognosis of LGG patients.