基于网络药理学的淫羊藿总黄酮治疗溃疡性结肠炎作用机制研究

Study on the mechanism of total flavonoids of Epimedium in the treatment of Ulcerative Colitis based on network pharmacology

目的基于网络药理学探讨淫羊藿总黄酮主要成分朝藿定A、朝藿定B、朝藿定C、淫羊藿苷、淫羊藿次苷对于溃疡性结肠炎治疗的作用机制。方法通过反向药效团匹配法,对淫羊藿总黄酮5种主要成分的潜在靶点进行预测,利用GeneCards数据库检索溃疡性结肠炎相关靶点,将以上两者取交集靶点进行蛋白互作,构建药物-成分-靶点-疾病网络,在DAVID数据平台做GO和KEGG富集分析。结果淫羊藿总黄酮的5种主要成分共得到249个潜在靶点,与溃疡性结肠炎有133个共同靶基因,其中有25个关键靶基因,GO富集分析共有三个部分共得到217个条目,KEGG富集分析得到87个条目,主要涉及癌症通路、磷脂酰肌醇-3-激酶/蛋白激酶B(phosphatidylinositol 3 hydroxykinase/protein kinase B,PI3K-Akt)信号通路等。结论网络药理学直观地展示了淫羊藿总黄酮可能从多成分、多靶点和多通路来治疗溃疡性结肠炎,为研究淫羊藿总黄酮抗溃疡性结肠炎的作用机制提供了科学依据。

Objective To explore the mechanism of the main components of total flavonoids of Epimedium(epimedin A,epimedin B,epimedin C,icariin and icarisideⅠ)in the treatment of Ulcerative Colitis based on network pharmacology.Methods The potential targets of the total flavonoids of Epimedium were predicted by the reverse pharmacophore matching method,and the related targets of ulcerative colitis were searched by the GeneCards database.The intersection targets were selected for protein interaction,and the drug-component-target-disease network was constructed.GO and KEGG enrichment analysis were done on the DAVID data platform.Results We found that the total flavonoids of Epimedium had 249 potential targets,which shared 133 common target genes with ulcerative colitis,including 25 key target genes.There were 217 items in three parts of GO enrichment analysis and 87 items in KEGG enrichment analysis,mainly related to cancer pathway,phosphatidylinositol 3 hydroxykinase/protein kinase B(PI3 K-Akt)signal pathway,and so on.Conclusion The network pharmacology directly shows that total flavonoids of Epimedium may treat ulcerative colitis from multi-components,multi-targets,and multi-pathways,which provides a scientific basis for studying the mechanisms of total flavonoids of Epimedium against ulcerative colitis.