葛根中内源性组分葛根素的生物效应

The biological effects of endogenous component puerarin in Radix Puerariae

探究了葛根中主要内源性组分葛根素的生物效应,为葛根的安全使用提供科学依据。将20只Balb/C健康小鼠按体重随机分为2组,即对照组和给药组,每组10只,雌雄各半。给药组小鼠一次性经口灌胃6000 mg•kg^(-1)的葛根素,对照组灌胃等体积溶剂聚乙二醇400,连续观察7 d,每天记录小鼠的一般活动状态、体重及死亡情况。7 d后小鼠经眼眶静脉处取血后处死,解剖观察小鼠心脏、肝脏、肾脏,并经HE染色后进行组织病理学研究。结果表明,葛根素雌雄小鼠的体重变化和心脏、肝脏和肾脏指数与对照组之间无显著性差异(P>0.05)。葛根素组雌性小鼠血清中尿素(Urea,UREA)水平显著高于对照组(P<0.05);葛根素组雌雄小鼠血清中乳酸脱氢酶(Lactic Dehydrogenase,LDH)和雄性大鼠血清中羟丁酸脱氢酶(Hydroxybutyric Dehydrogenase,HBDH)的含量极显著的低于对照组(P<0.05)。组织病理学研究表明,该剂量下的葛根素对小鼠的肝脏几乎不产生影响,但小鼠存在心肌溶解和肾小管间质局灶性出血。

The biological effects of puerarin,the main endogenous component in Radix Puerariae,were explored to provide a scientific basis for the safe use of Radix Puerariae.Twenty Balb/C healthy mice were randomly divided into two groups according to body weight,namely the control group and the medication group,with 10 mice in each group,half male and half female.The mice in the medication group were intragastrically administrated with puerarin at a dose of 6000 mg•kg^(-1) once,and the mice in the control group were intragastrically administrated with PEG 400 in the same volume.After 7 days of continuous observation,the general activity status,body weight and death of mice were recorded daily.Seven days later,the mice were sacrificed after blood was taken from the orbital vein.The heart,liver and kidney of the mice were anatomically observed and HE staining was performed for histopathological study.The results showed that there was no significant difference in the body weight change and heart,liver and kidney indexes between the male and female puerarin mice and the control group(P>0.05).Serum urea(UREA)level in puerarin group was significantly higher than that in control group(P<0.05);The levels of lactate dehydrogenase(LDH)in serum of male and female mice and hydroxybutyrate dehydrogenase(HBDH)in serum of male rats in puerarin group were significantly lower than those in the control group(P<0.05).Histopathological studies showed that puerarin had little effect on the liver of mice,while myocardial dissolution and focal renal tubulointerstitial hemorrhage were observed in mice.