镉胁迫下地钱转录组的性别特异性响应机制

Sex Specific Response Mechanism of Transcriptome in Both Male and Female Marchantia polymorpha Under Cadmium Stress

地钱(Marchantiapolymorpha)作为雌雄异株的苔纲植物,具有对重金属胁迫敏感的特性,且种群中雌雄比例差异较大,是研究重金属环境下植物性二态的理想对象之一。为探究雌雄地钱转录组对重金属镉的响应机制差异,利用高通量测序和加权基因共表达网络分析(WGCNA),鉴定了在镉胁迫条件下与不同性别地钱有关的模块和基因。结果表明,镉胁迫响应转录因子雌雄差异主要集中于bHLH、ERF和MYB家族。关键差异响应基因包括MARPO_0147s0038、MARPO_1326s0001和MARPO_0015s0058。差异响应通路雌性主要集中在信号转导通路,雄性则集中于类黄酮生物合成。研究结果有助于揭示雌雄异株地钱对镉胁迫的反应过程和响应机制,可为理解雌雄异株植物对重金属胁迫的性别特异性响应机制提供参考。

Marchantia polymorpha,a dioecious bryophyte,is characterized by hypersensitivity to heavy metal stress.There is a great difference in proportion of female and male in population,which means M.polymorpha is an ideal object to study plant sexual dimorphism under heavy metal stress.We used high-throughput sequencing and weighted gene co-expression network analysis(WGCNA)to explore the differences in the transcriptomes of M.polymorpha between male and female in response to the cadmium(Cd).We identified modules and genes associated with different sexes under Cd stress.The results showed that transcription factors differentially responsive to Cd stress were mainly the members of bHLH,ERF and MYB family,and some hub genes such as MARPO_0147s0038,MARPO_1326s0001,and MARPO_0015s0058.The differentially responsive pathways were mainly focused on signaling pathways in females and flavonoid biosynthesis pathway in males.These findings will help to reveal the process and mechanism of dioecious M.polymorpha response to Cd stress and provide a reference for understanding the sex-specific response mechanism of dioecious plants to heavy metal stress.