郑惠芳补肾健脾法对高龄体外受精-胚胎移植患者胚胎发育潜能的影响

Effect of ZHENG Huifang’s Tonifying Kidney and Invigorating Spleen on Embryonic Development Potential of Elderly in Vitro Fertilization-Embryo Transfer Patients

目的:探讨全国名老中医郑惠芳补肾健脾法对高龄体外受精-胚胎移植(IVF-ET)患者胚胎发育潜能的影响。方法:选取2019年8月至2020年8月于山东中医药大学生殖与遗传中心数据库中接受IVF-ET助孕治疗的100例高龄不孕患者(≥35岁)为研究对象,按照随机数字表法分为观察组和对照组各50例,两组患者均使用拮抗剂方案促排卵,观察组在对照组的基础上,加以郑惠芳补肾健脾经验方序贯治疗,共治疗3个月经周期。统计分析两组患者的年龄、不孕年限、获卵数、双原核受精率、可用胚胎数、优质胚胎数等资料,统计分析两组患者基础内分泌值及胚胎指标情况。结果:两组患者基础内分泌值比较差异无统计学意义(P>0.05)。治疗后,观察组在卵子获得数、双原核受精数、可用胚胎数均高于对照组(P<0.05);两组优质胚胎数比较差异无统计学意义(P>0.05),但观察组呈上升趋势。结论:补肾健脾法可促进不孕患者排卵,增加获卵数,改善胚胎发育潜能,提升高龄妇女IVF-ET过程可用胚胎数和优质胚胎数,可获取良好的妊娠结局。

Objective:To investigate the effect of ZHENG Huifang’s Tonifying Kidney and Invigorating Spleen on the embryonic development potential of elderly in vitro fertilization-embryo transfer(IVF-ET)patients. Method:A retrospective analysis was made on 100 elderly infertile patients (≥ 35 years old)who received IVF-ET in the database of the Reproductive and Genetic Center of Shandong University of Traditional Chinese Medicine from August 2019 to August 2020. The subjects were divided into the observation group and the control group with 50 cases in each according to the random number table method. Both groups received an antagonist program for ovulation induction,and the observation group received ZHENG Huifang’s Tonifying Kidney and Invigorating Spleen Formula for sequential treatment on the basis of the treatment of the control group,the treatment lasted a total of 3 menstrual cycles. The age,infertility years,number of eggs obtained,the number of double pronuclear fertilized embryos(2 PN),number of available embryos,number of high-quality embryos,other data of the two groups of patients were counted. Results:There was no significant difference in the baseline endocrine values between the two groups of patients(P>0.05). After treatment,the number of eggs obtained,the number of 2 PN fertilization,and the number of available embryos in the observation group were higher than those of the control group(P< 0.05);there was no significant difference in the number of high-quality embryos between the two groups(P>0.05),but the observation group showed an upward trend. Conclusions:Tonifying Kidney and Invigorating Spleen method can promote ovulation in infertile patients,increase the number of eggs obtained,improve the potential of embryonic development,and increase the number of embryos available and high-quality embryos in the IVF-ET process of elderly women,thereby obtaining a good pregnancy outcome.