抗菌肽RISE-AP12■对钛种植体周围炎主要致病菌和生物膜生长的抑制作用研究

Study on the inhibitory effect of antibacterial peptide RISE-AP12■on the main titanium peri-implantitis pathogens and the growth of biofilm

目的:探讨抗菌肽RISE-AP12■对钛种植体周围炎主要致病菌及其生物膜生长的抑制作用。方法:调整抗菌肽RISE-AP12■为不同的浓度,对种植体周围炎主要致病菌具梭核杆菌、血链球菌以及牙龈卟啉单胞菌的菌落进行监测,观察抗菌肽RISE-AP12■对主要致病菌的影响,确定最小抑菌浓度(MIC)与最小杀菌浓度(MBC),绘制抗菌肽RISE-AP12■作用于3种致病菌的kill-time曲线并观察其生物膜的变化。结果:抗菌肽RISE-AP12■对血链球菌、牙龈卟啉单胞菌、具梭核杆菌的MIC值分别为20、10、5 mg/L,MBC值分别为35、15、20 mg/L。根据kill-time曲线发现,在1 MIC和2 MIC时均具有较好的杀菌作用,2 MIC的抑菌作用显著强于1 MIC与阳性对照组(具核梭杆菌:t=1.653、1.763,P=0.013、0.008;牙龈卟啉单细胞菌:t=0.984、1.271,P=0.005、0.007;血链球菌:t=1.548、2.187,P=0.023、0.002)。当抗菌肽RISE-AP12■浓度为40 mg/L时,对生物膜的抑制作用最强,清除率显著高于阳性对照组(具核梭杆菌:t=2.768,P=0.032;牙龈卟啉单细胞菌:t=0.676,P=0.014;血链球菌:t=0.794,P=0.025)。结论:抗菌肽RISE-AP12■对钛种植体周围炎主要致病菌具梭核杆菌、血链球菌以及牙龈卟啉单胞菌均具有抑制作用,在一定浓度范围内,可以破坏其生物膜生长。

Objective:To investigate the inhibitory effect of antibacterial peptide RISE-AP12■on the main pathogens of titanium peri-implant inflammation and the growth of biofilm.Methods:The antimicrobial peptide RISE-AP12■was adjusted to different concentrations,the colonies of Fusobacterium nucleatum,Streptococcus sanguinis and Porphyromonasgingivalis were monitored,the effect of antimicrobial peptide RISE-AP12■on the main pathogens was observed,and the minimum inhibitory concentration(MIC)and minimum bactericidal concentration(MBC)were determined.The kill-time curves of antimicrobial peptide RISE-AP12■on three pathogens were drawn,and the changes of biofilm were observed.Results:The MIC values of antimicrobial peptide RISE-AP12■against Streptococcus sanguis,Porphyromonasgingivalis and Fusobacterium nucleatum were 20,10 and 5 mg/L,and the MBC values were 35,15 and 20 mg/L,respectively.According to the kill-time curve,it was found that both 1 MIC and 2 MIC concentrations had good bactericidal effect,and the bacteriostatic effect of 2 MIC concentration was significantly stronger than that of 1 MIC concentration and positive control group(Fusobacterium nucleatum:t=1.653,1.763,P=0.013,0.008;Porphyromonasgingivalis:t=0.984,1.271,P=0.005,0.007;Streptococcus sanguis:t=1.548,2.187,P=0.023,0.002).When the concentration of antimicrobial peptide RISE-AP12■was 40 mg/L,the inhibitory effect on biofilm was the strongest,and the clearance rate was significantly higher than that in the positive control group(Fusobacterium nucleatum:t=2.768,P=0.032;Porphyromonasgingivalis:t=0.676,P=0.014;Streptococcus sanguis:t=0.794,P=0.025).Conclusion:Antimicrobial peptide RISE-AP12■can inhibit the main pathogenic bacteria of titanium periimplant inflammation,such as Fusobacterium nucleatum,Streptococcus sanguis and Porphyromonasgingivalis.In a certain concentration range,it can destroy the growth of biofilm.