乳酸菌表层蛋白对其耐消化应激及肠道黏附能力的影响

Effect of Surface Layer Protein of Lactic Acid Bacteria on Its Tolerance of Oro-gastrointestinal Transit and Intestinal Adhesion

首先研究了乳酸菌的耐消化应激能力,然后研究了表层蛋白对菌株耐消化应激、黏附Caco-2细胞和抑制大肠杆菌黏附Caco-2细胞的影响。结果表明,依次经过唾液-胃液-肠液消化应激后,植物乳杆菌S7、W198和副干酪乳杆菌m111的存活率均大于3.53%,显著高于其他菌株(p<0.05),且菌株细胞均携带表层蛋白;去除表层蛋白后,菌株S7、W198和m111的耐消化应激能力显著下降(p<0.05),存活率较未去除的分别下降了12.08%、38.32%和2.35%;对Caco-2细胞的黏附率分别显著下降了6.64%、5.10%和14.43%(p<0.05)。菌株S7通过排斥、竞争和替代的方式来抑制大肠杆菌的黏附能力分别显著下降了4.22%、18.70%和12.06%(p<0.05);菌株W198通过排斥和替代的方式来抑制大肠杆菌黏附的能力显著下降了41.56%和22.20%(p<0.05);菌株m111通过排斥和取代的方式来抑制大肠杆菌黏附的能力分别显著下降了21.25%和30.40%(p<0.05)。植物乳杆菌S7、W198和副干酪乳杆菌m111具有较强的耐消化应激能力,表层蛋白能增强其耐消化应激及黏附肠道的能力。

The surface(S)layer protein of lactic acid bacteria(LAB)has the ability of improving the survival rate,increasing adhesion,and regulating immunity and many other kinds of beneficial function.The aim of this study was to evaluate the resistance to oro-gastrointestinal transit(OGT)stress of LAB,and the effect of S-layer protein on LAB resistant OGT stress and its adhesion of Caco-2 cells and the inhibition of Escherichia coli(E.coli)adhesion to Caco-2 cells were also studied.The results showed that the survival rates of Lactobacillus plantarum S7,W198 and Lactobacillus paracasei m111 were more than 3.53%after passing simulated saliva,gastric juice and intestinal juice successively,and significantly higher than those of others(p<0.05),which have the S-layer protein.The resistance of S7,W198 and m111 to OGT stress decreased significantly(p<0.05),and the survival rate decreased by 12.08%,38.32%and 2.35%respectively after their S-layer protein were removed,and the adhesion rates to Caco-2 cells decreased significantly by 6.64%,5.10%and 14.43%respectively(p<0.05).The ability of S7 to inhibite the adhesion of E.coli on the intestinal by exclusion,competition and substitution,and the inhibition rate was significantly reduced by 4.22%,18.70%and 12.06%respectively(p<0.05).The ability of W198 to inhibite the adhesion of E.coli on the intestinal by exclusion and substitution,and the inhibition rate was significantly reduced by 41.56%and 22.20%respectively(p<0.05).The ability of m111 to inhibite the adhesion of E.coli on the intestinal by exclusion and substitution,and the inhibition rate was significantly reduced by 21.25%and 30.40%respectively(p<0.05).L.plantarum S7,W198 and L.paracasei m111 have strong resistance to OGT stress,and S-layer proteincan enhance their OGT stress and intestinal adhesion.