基于石墨烯固相萃取-毛细管电泳联用的奶嘴中痕量双酚A检测

Detection of trace bisphenol A in nipple based on graphene solid phase extraction coupled with capillary electrophoresis

制备高比表面积的石墨烯纳米材料,然后将其用做固相萃取(SPE)吸附剂制备石墨烯基SPE小柱。利用所制备的石墨烯基SPE小柱对双酚A(BPA)的高效富集作用并结合毛细管(CE)技术的高效检测能力构建SPE-CE联用分析方法。通过优化实验获取所制备的石墨烯基SPE小柱对BPA的最优萃取条件和最优CE检测条件。在最优条件下,经与商品化的SPE小柱对比,所构建的SPE-CE联用分析方法对BPA在0.05~80μg/mL范围内表现出良好的线性关系,检出限可达0.02μg/mL(S/N=3σ)。此外,采用甲醇超声提取和纯水回流提取两种方法提取婴儿奶嘴实际样品中痕量BPA,用所构建的SPE-CE联用分析方法测定婴儿奶嘴实际样品中BPA的迁移量,该方法表现出良好的准确性和精密度,可实现实际样品中痕量BPA的高灵敏快速检测。因此,该文所构建的联用方法可为食品接触材料中痕量组分的分析检测提供可行的思路,具有广阔的应用前景。

High specific surface area graphene was first prepared and used as the solid phase extraction(SPE)adsorbent to prepare the graphene based SPE tube.Based on the high efficiency adsorption capacity for bisphenol A(BPA)of graphene and the fine detection ability of capillary electrophoresis(CE),the SPE-CE coupling technique was constructed.Through the optimization of experiment,the optimal SPE experimental condition and CE detection condition was acquired.Under the optimal experimental condition,compared with the commercial SPE tube,the constructed SPE-CE coupling method exhibited fine line relationship between 0.05-80μg/mL with a detection limit of 0.02μg/mL(S/N=3σ)for BPA detection.Furthermore,for real infant nipple sample BPA detection,methanol ultrasonic extraction and water reflux extraction were used to extract the trace BPA in infant nipple.Then,the SPE-CE coupling method was used for the detection of BPA migration mass of infant nipple.It shown fine accuracy and precision which exhibited that the constructed SPECE coupling method can achieved the fast and sensitive detection of trace BPA in real sample.Thus,this detection strategy provided a feasible approach for trace component in food contacting material and shown wide application prospect.