HPLC-CAD同时测定复方醋酸钠林格注射液中钠、钾、镁、钙的含量

Simultaneous determination of sodium,potassium,magnesium and calcium in compound sodium acetate Ringer injection by HPLC-CAD

该文建立一种HPLC-CAD方法同时测定复方醋酸钠林格注射液中钠、钾、镁、钙离子的含量。采用SiELC Primesep A柱,以0.1%三氟乙酸水溶液-0.25%三氟乙酸乙腈为流动相,梯度洗脱,流量1 mL/min,柱温30℃,电雾式检测器(CAD)参数:数据采集频率10 Hz,滤波5 s,雾化器温度50℃。检测结果表明钠、钾、镁、钙的线性范围分别为3~60μg/mL、3~60μg/mL、0.5~10μg/mL、1.2~24μg/mL,线性关系良好(n=6)。平均回收率分别为101.7%、98.41%、99.28%、99.73%(n=9)。该文建立的HPLC-CAD方法可用于复方醋酸钠林格注射液中的钠、钾、镁和钙离子的含量测定,研究结果可为复方醋酸钠林格注射液的质量评价提供新方法。

To establish a HPLC-CAD method to simultaneously determine the content of sodium,potassium,magnesium,calcium in compound sodium acetate Ringer injection.The analysis was performed on SiELC Primesep A column with 0.1%trifluoroacetic acid aqueous solution and 0.25%trifluoroacetic acid acetonitrile as the mobile phase under gradient elution.The flow rate was 1 mL/min,the column temperature was set at 30℃.CAD parameters was 10 Hz for sampling rate,50℃for the nebulizer temperature,and filter for 5 s.The linear ranges of sodium,potassium,magnesium and calcium were 3-60μg/mL,3-60μg/mL,0.5-10μg/mL,1.2-24μg/mL,respectively(n=6).The average recoveries were 101.7%,98.41%,99.28%,99.73%,respectively(n=9).The established HPLC-CAD method can be used for the determination of sodium,potassium,magnesium and calcium ions in compound sodium acetate Ringer injection.The research results provide a new method for the quality evaluation of compound sodium acetate Ringer injection.