老年血管性痴呆患者血清8-羟基脱氧鸟苷和孤独G蛋白偶联受体配体-13水平与病情严重程度及预后的相关性分析

Correlation analysis of the levels of serum 8-hydroxy-2 deoxyguanosine and solitary G protein-coupled receptor ligand-13 with disease severity and prognosis in elderly patients with vascular dementia

目的探究老年血管性痴呆(Vascular dementia,VD)患者血清8-羟基脱氧鸟苷(8-hydroxy-2 deoxyguanosine,8-OHdG)、孤独G蛋白偶联受体配体-13(Solitary G Protein-Coupled Receptor ligand-13,Apelin-13)蛋白水平变化及其与患者病情严重程度、预后的关系。方法选取西部战区总医院2017年12月—2020年12月收治的125例老年VD进展期患者为观察组,同期健康体检者92例为对照组。2组均给予简易精神状态检查表(Mini-mental state examination,MMSE)评分;观察组根据MMSE评分结果分为轻度亚组、中度亚组、重度亚组。比较观察组、对照组及观察组各亚组间血清8-OHdG、Apelin-13蛋白水平及MMSE评分;采用Pearson方法分析血清8-OHdG、Apelin-13蛋白水平与MMSE评分的相关性;观察组患者出院后3个月,根据ADL评分,分为Ⅰ级组、Ⅱ级组、Ⅲ级组,比较各级组间血清8-OHdG、Apelin-13蛋白水平,采用Spearman方法分析老年VD患者血清8-OHdG、Apelin-13蛋白水平与预后的关系。结果不同病情严重程度组间血清8-OHdG、Apelin-13蛋白水平及MMSE评分比较差异均有统计学意义(P<0.05);Pearson相关分析表明,老年VD患者血清8-OHdG水平与MMSE评分呈负相关(P<0.05),而Apelin-13与MMSE评分呈正相关(P<0.05);不同ADL分级组间血清8-OHdG、Apelin-13蛋白水平差异有统计学意义(P<0.05);Spearman相关分析提示,老年VD患者血清8-OHdG与预后(ADL分级)呈正相关(P<0.05),Apelin-13水平与预后(ADL分级)呈负相关(P<0.05)。结论老年VD患者患者血清8-OHdG异常升高,而Apelin-13蛋白水平降低,临床可能用于VD患者病情严重程度及预后的评估。

Objective To explore serum 8-hydroxy-2 deoxyguanosine(8-OHDG)and Solitary G protein-coupled receptor ligand 13(Solitary G protein-coupled)in senile patients with Vascular dementia(VD)Receptor ligand-13,Apelin-13 protein level and its relationship with disease severity and prognosis.Methods from December 2017 to December 2020,125 elderly patients with advanced VD were selected as the observation group,and 92 healthy subjects during the same period were selected as the control group.Both groups were given mini-Mental State Examination(MMSE)scores.The observation group was divided into mild subgroup,moderate subgroup and severe subgroup according to MMSE score.The serum 8-OHDG and Apelin-13 protein levels and MMSE scores of observation group,control group and observation group were compared.The correlation between serum 8-OHDG and Apelin-13 protein levels and MMSE score was analyzed by Pearson method.Three months after discharge,observation group was divided into gradeⅠgroup,gradeⅡgroup and gradeⅢgroup according to ADL score.The serum 8-OHDG and Apelin-13 protein levels were compared among all groups.Spearman method was used to analyze the relationship between serum 8-OHDG and Apelin-13 protein levels and prognosis in elderly PATIENTS with VD.Results There were statistically significant differences in serum 8-OHDG and Apelin-13 protein levels and MMSE score among different severity groups(P<0.05).Pearson correlation analysis showed that serum 8-OHDG level was negatively correlated with MMSE score in elderly PATIENTS with VD(P<0.05),while apelin-13 was positively correlated with MMSE score(P<0.05).There were statistically significant differences in serum 8-OHDG and Apelin-13 protein levels among different ADL grading groups(P<0.05).Spearman correlation analysis suggested that serum 8-OHDG was positively correlated with prognosis(ADL grade)in elderly patients with VD(P<0.05),while apelin-13 level was negatively correlated with prognosis(ADL grade)(P<0.05).Conclusion Serum 8-OHDG is abnormally elevated and apelin-13 protein level is decreased in elderly patients with VD,which may be used to evaluate the severity and prognosis of VD patients.