超声辅助酶法提取黔产青钱柳多糖

Ultrasound-assisted Enzymatic Extraction of Polysaccharide from Cyclocarya paliurus from Guizhou

以黔产青钱柳为原料,采用超声辅助酶法提取青钱柳多糖,以青钱柳多糖含量为指标,考察酶添加量、酶解pH值、超声时间等因素对青钱柳多糖的影响,采用单因素试验与正交试验优化提取工艺。结果表明,最佳条件为酶添加量4.5%、酶解pH=6.0、酶解超声时间30 min,此时青钱柳多糖含量为16.23%。采用超声辅助酶法提取青钱柳多糖,提取工艺稳定,提取效率高,可为青钱柳进一步开发利用奠定基础。

Taking the Cyclocarya paliurus produced in Guizhou as the raw material,the polysaccharide of Cyclocarya paliurus was extracted by ultrasonic-assisted enzymatic method,and the polysaccharide content of Cyclocarya paliurus was used as the index to investigate the effects of the amount of enzyme added,the pH value of enzymatic hydrolysis,the ultrasonic time and other factors on the polysaccharide of Cyclocarya paliurus,using single factor test and orthogonal test to optimize the extraction process.The results showed that the optimum conditions were 4.5%enzyme addition,pH=6.0 for enzymatic hydrolysis,and 30 min ultrasonic time for enzymatic hydrolysis,at which time the polysaccharide content of Cyclocarya paliurus was 16.23%.Using ultrasonic-assisted enzymatic method to extract the polysaccharide of Cyclocarya paliurus,the extraction process is stable and the extraction efficiency is high,which can lay a foundation for the further development and utilization of Cyclocarya paliurus.