胃癌组织中脂肪酶H的表达情况及临床意义

Expression of lipase member H in gastric cancer and its clinical significance

目的研究胃癌中脂肪酶H(LIPH)的表达情况及其临床意义。方法选取正常胃黏膜细胞GES-1和胃癌细胞株SGC-7901、AGS、MGC-803、BGC-823、MKN-45,通过Western blot检测LIPH在其中的表达情况。选取2014年1月至2015年12月蚌埠医学院第一附属医院收治的95例胃癌患者,采用Western blot和免疫组织化学法检测LIPH在其癌组织和癌旁组织中的表达水平。根据LIPH表达情况将其分为阳性组和阴性组,对两组进行60个月的随访,采用生存曲线和Cox回归分析LIPH在胃癌中的临床意义。结果胃癌细胞株MGC-803、AGS、MKN-45、BGC-823、SGC-7901的LIPH表达高于正常胃黏膜细胞GES-1,差异有统计学意义(P<0.05)。免疫组化结果显示,胃癌组织中LIPH阳性表达呈棕色或棕褐色。癌组织中LIPH表达阳性率高于癌旁组织,差异有统计学意义(P<0.05)。95例患者累积生存率为22.1%,阳性组(56例)累积生存率低于阴性组(39例),差异有统计学意义(P<0.05)。单因素分析结果显示LIPH表达、浸润深度、淋巴结转移情况、TNM分期、分化程度均与胃癌预后相关(P<0.05)。多因素分析结果显示LIPH表达阴性、浸润深度T1~T2、无淋巴结转移、TNM分期Ⅰ期均为胃癌患者预后保护因素(HR<1,P<0.05)。结论LIPH在胃癌中高表达,与胃癌的预后相关。

Objective To study the expression of lipase member H(LIPH)in gastric cancer and its clinical significance.Methods Normal gastric mucosa cells GES-1 and gastric cancer cell lines SGC-7901,AGS,MGC-803,BGC-823,and MKN-45 were selected,and the expression of LIPH in them was detected by Western blot.A total of 95 gastric cancer patients admitted to the First Affiliated Hospital of Bengbu Medical College from January 2014 to December 2015 were selected.Western blot and immunohistochemistry were used to detect LIPH expression levels in cancer tissues and adjacent tissues.LIPH was divided into positive group and negative group according to the expression of LIPH.The two groups were followed up for 60 months,and the clinical significance of LIPH in gastric cancer was analyzed by survival curve and Cox regression.Results The LIPH expression of gastric cancer cell lines MGC-803,AGS,MKN-45,BGC-823,and SGC-7901 was higher than that of normal gastric mucosa cells GES-1,and the differences were statistically significant(P<0.05).Immunohistochemical results showed that LIPH positive expression in gastric cancer tissues was brown or tan.The positive rate of LIPH expression in cancer tissues was higher than that in adjacent tissues,and the difference was statistically significant(P<0.05).The cumulative survival rate of 95 patients was 22.1%,the cumulative survival rate of positive group(56 cases)was lower than that of negative group(39 cases),the difference was statistically significant(P<0.05).Univariate analysis showed that LIPH expression,invasion depth,lymph node metastasis,TNM stage,and differentiation degree were correlated with prognosis of gastric cancer(P<0.05).Multivariate analysis showed that negative LIPH expression,invasion depth T1-T2,no lymph node metastasis,and TNM stageⅠwere prognostic protective factors for gastric cancer patients(HR<1,P<0.05).Conclusion LIPH is highly expressed in gastric cancer,which is related to the prognosis of gastric cancer.