基于网络药理学分析中医药防治宫腔粘连的作用机制

Mechanism analysis of traditional Chinese medicine prevention and treatment of intrauterine adhesions based on network pharmacology

目的凭借网络药理学方法,探讨中医药治疗宫腔粘连(IUA)作用靶点及机制。方法检索发表在中国知网等数据库中有关中医药治疗IUA的数据挖掘类文献,对涉及的高频次中药进行统计,采用网络药理学分析高频中药治疗IUA的作用机制,并通过分子对接技术进行验证。结果文献中排名前6位的高频中药是当归、熟地黄、川芎、桃仁、红花、牛膝。网络药理学分析得出包括细胞肿瘤抗原p53(TP53)、信号传导与转录激活因子3(STAT3)、丝裂原活化蛋白激酶1(MAPK1)等的64个核心靶点,主要涉及细胞凋亡过程、细胞增殖调控、药物反应等生物过程,胞质、核浆、细胞外区域等细胞结构,蛋白结合、酶结合、转录因子结合等分子功能以及T细胞受体信号通路、PI3K-Akt信号通路、Toll样受体信号通路等信号通路。分子对接显示核心药物化学成分与核心靶点均有较好的结合能力。结论高频中药防治IUA可能通过调控TP53、STAT3、MAPK1等核心靶标,激活T细胞受体信号通路、PI3K-Akt信号通路等起到调节内膜细胞周期、局部炎症-免疫调节等发挥治疗作用。

Objective To explore the target and mechanism of traditional Chinese medicine in the treatment of intrauterine adhesions(IUA)by network pharmacology.Methods Data mining literatures related to traditional Chinese medicine treatment of IUA published in CNKI and other databases were searched to make statistics of the high-frequency traditional Chinese medicine involved,and the mechanism of action of high-frequency traditional Chinese medicine treatment of IUA was analyzed by network pharmacology,and verified by molecular docking technology.Results The top 6 high-frequency traditional Chinese medicines in the literature were Angelica sinensis,Rehmannia glutinosa,Ligusticum wallichii,peach kernel,safflower and Achyranthes bidentata.Network pharmacology analysis including cell p53 tumor antigen(TP53),signal transduction and transcriptional activation factor 3(STAT3),silk crack the original activated protein kinase 1(MAPK1)and other 64 core targets,mainly involves the process of cell apoptosis,cell proliferation regulation,drug reactions,such as biological processes,cytoplasm,nuclear pulp,extracellular cell structures such as area,protein binding,enzyme binding,transcription factor binding and other molecular functions and T cell receptor signaling pathway,PI3K-Akt signaling pathway,Toll-like receptor signaling pathway and other signaling pathways.The molecular docking showed that the core drug chemical components had good binding ability with the core target.Conclusion High-frequency traditional Chinese medicine treatment of IUA may play a therapeutic role by regulating TP53,STAT3,MAPK1 and other core targets,activating T cell receptor signaling pathway and PI3K-Akt signaling pathway to regulate endometrial cell proliferation and differentiation,local inflammation and immune regulation.