摘要
目的应用全基因组测序(WGS)和脉冲场凝胶电泳(PFGE)分型技术鉴别新疆一起疑似聚集性伤寒疫情。方法通过新疆维吾尔自治区传染病报告系统收集5例伤寒病例,对5例病例的流行病学史进行回顾性调查,重点针对病例的诊断过程以及疑似感染来源进行调查,同时对5株分离到的伤寒沙门菌进行WGS和PFGE分析和聚类,最后结合流行病学调查结果,判定疫情性质。结果5株伤寒沙门菌的基因组序列、PFGE图谱、耐药表型和耐药基因高度一致;流行病学调查发现,5例病例曾于同一天在同一家餐馆就餐。结论结合伤寒最长潜伏期、病原菌分子分型和流行病学调查结果,可初步判定此次疫情为一起通过就餐食物而感染伤寒沙门菌的聚集性疫情。
Objective To identify a suspected clustered Typhoid fever by whole genome sequencing(WGS)and pulsed field gel electrophoresis(PFGE)subtyping.Methods The nature of the epidemic was determined by combination of subtyping results of isolates and epidemiological information.Results Five S.typhimurium isolates showed identical PFGE patterns and almost the same whole genome sequence.Epidemiological survey showed that five cases had dined in the same restaurant on the same day.Conclusion Combined with the longest incubation period of typhoid fever,molecular subtyping of pathogenic bacteria and the field epidemiological survey,it can be preliminarily determined that the five cases have common infection sources.
作者
刘艳
马合木提
李方
夏依旦·吾甫尔
周海健
Liu Yan;Mahe Muti;Li Fang;Xiayidan·Wufuer;Zhou Haijian(Infectious Disease Prevention and Control Department,Center for Disease Control and Prevention of Xinjiang Uygur Autonomous Region,Urumqi 830002,China;State Key Laboratory of Infectious Disease Prevention and Control,National Institute for Communicable Disease Control and Prevention,Chinese Center for Disease Control and Prevention,Beijing 102206,China)
出处
《中华预防医学杂志》
CAS
CSCD
北大核心
2022年第4期433-436,共4页
Chinese Journal of Preventive Medicine
基金
国家重点研发计划(2018YFC1603804)。
关键词
伤寒
沙门菌
伤寒
基因组
细菌
脉冲场凝胶电泳
全基因组测序
Typhoid fever
Salmonella typhi
Genome,bacterial
Pulsed field gel electrophoresis
Whole genome sequencing
