不同基因型茶树品种对稀土、氟和铝积累响应的转录分析

Transcriptional Analysis for the Response of Different Genotypes of Tea Plants to Rare Earth,Fluoride and Aluminum Accumulation

茶(Camellia sinensis(L.)O.Kuntze)是一种天然保健饮料,在世界饮料史上起着极其重要的作用。从种质资源的角度来看,不同茶树品种/种质间稀土元素(REE)、氟(F)和铝(Al)含量的差异达显著水平。本研究以前期鉴定出的REE、F和Al的低积累(T15)、中积累(本山)和高积累(水仙)的不同基因型茶树品种为材料,基于Illumina平台的转录组测序,分析差异基因表达情况。结果显示通过转录组测序共发现了83357条基因,GO功能分类到44个类别中,主要涉及细胞、细胞器、细胞膜和大分子复合物;不同茶树种质间富集最显著的代谢通路为植物激素信号转导、谷胱甘肽代谢,随机选择的8个基因实时荧光定量(Q-PCR)结果进一步证实了RNA测序结果。同时ABC转运蛋白可能参与吸收和富集。这些新发现为低REE、F和Al积累茶树品种的筛选和培育提供了理论基础。

Tea(Camellia sinensis(L.)O.Kuntze)is a kind of natural health drink,which has played an extremely important role in the world’s beverage history.In terms of germplasm resources,there were significant differences in REE,fluorine(F)and aluminum(Al)contents among different tea plant varieties.In this study,taking low(T15),medium(Benshan)and high(Shuixian)enrichment tea plants of REE,F and Al as the research objects.Send the above samples for sequencing.Through the GO function classification,in the tea Unigenes,18967 differences were classified into 44 functional categories.Gene set pathway enrichment analysis indicated that the digital gene expression are predominantly involved in the plant hormone signal transduction,Glutathione metabolism.Q-PCR results of 8 randomly selected DEGs affirmed the RNA-sequencing results.In addition,ABC transporter might participate in the positively regulation of metal elements absorption and accumulation behavior.These new findings provide theoretical basises for the screening and cultivation of low REE,F and Al accumulative tea plant varieties.