摘要
谷子刚毛为未发育的小穗退化而成,其中长刚毛对减轻和防止鸟害有重要的作用,然而目前控制谷子刚毛发育的分子遗传机制仍不是很清楚。本研究以长刚毛品种‘毛毛谷’为父本和短刚毛品种‘晋谷21’为母本构建了谷子刚毛长短的F_(2)杂交群体,利用BSA方法和转录组测序技术,进行刚毛长度相关QTL定位,并在该区间内挖掘候选基因。结果发现,控制刚毛长度的QTL定位在1号染色体35~40 Mb的基因组区间内,Seita.1G327200和Seita.1G279300为可能的候选基因。本研究初步解析了谷子刚毛发育的分子机制,为谷子种质资源改良奠定理论基础。
Foxtail millet bristle is degenerated from undeveloped spikelet and long bristle has the effect of reducing and preventing bird feeding.But little is known about the mechanism of bristle development.In this study,we constructed a F_(2) hybrids population using the long-bristled’Maomaogu’as a male parent and the short-bristled’Jingu21’as a female parent.By using the F_(2) hybrids population with a new method based on bulked segregant analysis,This research performed QTL mapping and searched candidate genes for bristle length.Our results showed that a QTL for bristle length was located in the 35~40 Mb of chromosome 1,and Seita.1G327200 and Seita.1G279300were the potential candidate genes for this trait.This project could provide a theoretical basis for the molecular mechanism of bristle development and the germplasm improvement in foxtail millet.
作者
贾娅楠
李可扬
贾举庆
张艾英
张莉
郭世华
Jia Yanan;Li Keyang;Jia Juqing;Zhang Aiying;Zhang Li;Guo Shihua(College of Agriculture,Shanxi Agricultural University,Taigu,030801;Millet Research Institute,Shanxi Academy of Agricultural Sciences,Changzhi,046011;Agricultural College,Inner Mongolia Agricultural University,Hohhot,010019)
出处
《分子植物育种》
CAS
北大核心
2022年第7期2335-2340,共6页
Molecular Plant Breeding
基金
山西农业大学省部共建有机旱作农业国家重点实验室自主研发项目(202105D121008-2-5)
山西农业大学生物育种工程项目(YZGC029)共同资助国家自然科学基金项目(31860409,32060509)
国家重点研发项目(2020YFD1000803-2)
山西省应用基础研究计划项目(201901D111221)。
关键词
谷子
刚毛长度
遗传分析
QTL定位
Foxtail millet
Bristle length
Genetic analysis
QTL mapping
