宫颈鳞状细胞癌组织核糖体结合蛋白1、E2F转录因子1的表达及其临床意义

Expression of ribosome-binding protein 1 and E2F transcription factor 1 in cervical squamous cell carcinoma and its clinical significance

目的探讨宫颈鳞状细胞癌(cervical squamous cell carcinoma,CSCC)组织中核糖体结合蛋白1(reticulum ribosome-binding protein 1,RRBP1)、E2F转录因子1(E2F transcription factor 1,E2F-1)的表达与临床价值。方法选取山西省肿瘤医院2017年7月至2018年1月收治的CSCC患者83例作为CSCC组,另选取同期于我院行宫颈活检的慢性宫颈炎患者80例作为对照组。采用免疫组化法检测两组病变组织中RRBP1、E2F-1表达情况,分析二者与CSCC患者临床病理特征的关系。绘制Kaplan-Meier曲线并采用Log-rank检验比较不同RRBP1、E2F-1表达患者术后3年的生存情况。多因素Cox回归分析CSCC患者预后的影响因素。结果CSCC组RRBP1、E2F-1阳性表达率分别为74.70%、54.22%,高于对照组的18.75%、13.75%,差异有统计学意义(P<0.05)。不同FIGO分期、分化程度、淋巴结转移、肿瘤大小、脉管浸润、人乳头瘤病毒感染情况的CSCC患者癌组织中RRBP1、E2F-1表达情况对比差异有统计学意义(P<0.05)。RRBP1阳性组总生存率低于RRBP1阴性组,且E2F-1阳性组无瘤生存率、总生存率低于E2F-1阴性组(P<0.05)。多因素Cox回归分析结果提示FIGO分期、分化程度、淋巴结转移、脉管浸润、HPV感染、RRBP1与E2F-1表达是CSCC患者预后的影响因素(P<0.05)。结论CSCC患者癌组织中RRBP1、E2F-1阳性表达率异常升高,二者阳性表达率升高会降低患者术后总生存率,是其预后的影响因素。

Objective To investigate the expression and clinical relationship of reticulum ribosome-binding protein 1(RRBP1) and E2 F transcription factor 1(E2 F-1) in cervical squamous cell carcinoma(CSCC) tissue value.Methods A total of 83 CSCC patients admitted to Shanxi Cancer Hospital from July 2017 to January 2018 were selected as the CSCC group, and 80 patients with chronic cervicitis who underwent cervical biopsy in our hospital during the same period were selected as the control group.Immunohistochemical method was used to detect the expressions of RRBP1 and E2 F-1 in the lesions of the two groups, and the relationship between the two and the clinicopathological characteristics of CSCC patients were analyzed.The Kaplan-Meier curve was drawn and the Log-rank test was used to compare the 3-year survival of patients with different RRBP1 and E2 F-1 expressions.Multivariate Cox regression analysis of prognostic factors of CSCC patients.Results The positive expression rates of RRBP1 and E2 F-1 in CSCC group were 74.70% and 54.22%,respectively, which were higher than 18.75% and 13.75% in control group(P<0.05).There were significant differences in the expression of RRBP1 and E2 F-1 in cancer tissues of CSCC patients with different FIGO stages, degrees of differentiation, lymph node metastasis, tumor size, vascular invasion, and human papillomavirus infection(P<0.05).The overall survival rate of the RRBP1 positive group was lower than that of the RRBP1 negative group, and the tumor-free survival rate and overall survival rate of the E2 F-1 positive group were lower than those of the E2 F-1 negative group(P<0.05).Multivariate Cox regression analysis showed that FIGO stage, degree of differentiation, lymph node metastasis, vascular invasion, HPV infection, RRBP1 and E2 F-1 expression were prognostic factors in CSCC patients(P<0.05).Conclusion The positive expression rates of RRBP1 and E2 F-1 in cancer tissues of CSCC patients are abnormally increased, and the increased positive expression rates of the two can reduce the overall survival rate of patients after surgery, and are the prognostic factors.