摘要
芽孢杆菌Ya-1对辣椒枯萎病有较好的防治效果。为明确其是否具有诱导辣椒抗枯萎病的能力,本研究采用“浸根+灌根”法接种辣椒幼苗,分析不同处理前后辣椒叶片苯丙氨酸解氨酶(PAL)、过氧化物酶(POD)、超氧化物歧化酶(SOD)的活性及丙二醛(MDA)含量的变化。同时,RT-PCR分析了抗性相关蛋白PR-1、POD酶和SOD酶合成相关基因POD和SOD1的转录表达情况。结果表明,Ya-1处理后辣椒叶片PAL、POD和SOD活性较处理前显著上升,而MDA含量显著下降;抗性相关蛋白PR-1、防御酶相关基因POD和SOD1表达水平明显上调。本研究说明诱导辣椒产生抗病性是Ya-1防治辣椒枯萎病的机理之一。
Bacillus sp.Ya-1 could suppress Fusarium wilt of pepper.In order to explore the potential mechanism underlying the induced resistance mediated by Ya-1,pepper seedlings were inoculated with’root immersion and root irrigation’method in this study.And the changes of activities of phenylalanine ammonia-lyase(PAL),peroxidase(POD),superoxide dismutase(SOD)and malondialdehyde(MDA)content in pepper leaves before and after different treatments were analyzed.At the same time,RT-PCR was used to analyze the transcriptional expression of several enzymes synthesis-related genes,POD,SOD1 and defense-related gene PR-1.The results showed that levels of SOD,POD,PAL activities and SOD1,POD,PR-1 were all significantly higher than those of the control,while the level of MDA was significantly lower.The expression level of PR-1,POD and SOD1 were significantly enhanced after root immersion and root irrigation with Ya-1 fermentation broth.The above results suggested that the induced resistance in pepper by Ya-1 might be one of the mechanisms to control pepper Fusarium wilt caused by F.oxysporum.
作者
赵志祥
严婉荣
王宝
陈圆
王会芳
肖彤斌
Zhao Zhixiang;Yan Wanrong;Wang Bao;Chen Yuan;Wang Huifang;Xiao Tongbin(Hainan Key Laboratory for Control of Plant Diseases and Insect Pests,Scientific Observation and Experiment Station of Crop Pests in Haikou,Ministry of Agriculture,Research Center for the Quality Safety and Standards of Agricultural Products in Hainan Academy of Agricultural Sciences,Institute of Plant Protection of Hainan Academy of Agricultural Sciences,Haikou,571100)
出处
《分子植物育种》
CAS
北大核心
2022年第8期2699-2706,共8页
Molecular Plant Breeding
基金
海南省自然科学基金创新研究团队项目(2019CXTD403)
省属科研院所技术开发专项(JBKYYWF-2021)共同资助。
关键词
芽孢杆菌
防御酶
诱导抗病性
抗病基因
Bacillus sp.
Defense-related enzymes
Induced resistance
Resistance-related genes