溃疡性结肠炎相关性结肠癌与偶发性结直肠癌的差异基因分析

Differential genes analysis between ulcerative colitis-associated colorectal cancer and sporadic colorectal cancer

目的基于GEO数据库查找溃疡性结肠炎相关性结肠癌与偶发性结直肠癌的数据集,得到差异基因,分析其对预后的影响,为肿瘤的个体化治疗寻求方法。方法通过GEO数据库下载两种类型肿瘤数据集,然后导入R 4.0.3软件,获得表达矩阵,分析差异基因,并进行GO和KEGG分析,采用STRING数据库和Cytoscape软件分析蛋白质相互作用,识别中枢基因。GEPIA网站验证中枢基因是否具有表达差异以及对预后的影响。结果溃疡性结肠炎相关性结肠癌和偶发性结直肠癌共筛选出上调基因396个,下调基因141个。蛋白质相互作用网络中筛选出10个关联度较高的中枢基因,其中CCL4、CCL2、IL1B、ITGAM基因的表达与预后有关。结论溃疡性结肠炎相关性结肠癌与偶发性结直肠癌差异基因中CCL4、CCL2、ITGAM对预后影响明显,IL1B基因的表达与预后相关。

Objective To find the datasets of ulcerative colitis-associated colorectal cancer(UC-CRC)and sporadic colorectal cancer(CRC)based on GEO database,obtain the differential genes and analyze their prognostic impact to seek methods for individualized treatment of tumors.Methods The datasets of ulcerative colitis-associated colorectal cancer and sporadice colorectal cancer were downloaded from GEO database,then imported them into R 4.0.3software to obtain expression matrices for differential analysis,followed by GO and KEGG analysis,STRING database and Cytoscape software were used for protein interactions to identify hub genes.GEPIA website was used to verify whether the hub genes were differentially expressed and their prognostic impact.Results 396 up-regulated genes and 141 down-regulated genes were abtained in ulcerative colitis-associated colorectal cancer and sporadic colorectal cancer.10 hub genes with high association were selected,among which the expression of CCL4,CCL2,IL1B,ITGAM genes were associated with prognosis.Conclusion Among differential expressed genes of ulcerative colitis-associated colorectal cancer and sporadic colorectal cancer,the expressions of CCL4,CCL2,ITGAM genes significantly impact prognosis,and the expression of IL1B gene is correlated with prognosis.