针刀对膝骨关节炎兔股直肌组织硬度和纤维化的影响

Effect of akupotomye on stiffness and fibrosis of rectus femoris muscle in rabbits with knee osteoarthritis

目的探讨针刀干预对膝骨关节炎(KOA)兔股直肌组织硬度和纤维化的影响。方法将24只健康雄性新西兰兔采用随机数字表法分为空白组、模型组和针刀组,每组8只。改良Videman法左后肢伸直位石膏固定6周制备KOA兔模型,以膝关节行为学Lequesne MG评分≥4分为模型成功标准。针刀组对“鹤顶次”“髌外上”“髌内下”行针刀松解治疗,每7天干预1次,共干预4次。空白组、模型组做同样的抓取和固定,但不予针刀治疗。干预结束1周后,行实时组织弹性成像检测弹性应变率比值评估股直肌组织硬度改变;Masson染色观察股直肌组织形态及胶原纤维含量改变。结果与空白组比较,模型组股直肌弹性应变率比值及胶原容积分数均升高(P<0.05);与模型组比较,针刀组股直肌弹性应变率比值及胶原容积分数均下降(P<0.05)。结论针刀干预可以改善KOA兔股直肌组织硬度,促进股直肌慢性损伤纤维化的修复,这可能是针刀治疗KOA的作用机制之一。

Objective To explore the effect of akupotomye on stiffness and fibrosis of rectus femoris muscle in rabbits with knee osteoarthritis(KOA).Methods Twenty-four healthy male New Zealand rabbits were divided into blank group,model group,and akupotomye group by random number table method,with eight rabbits in each group.The KOA rabbit model was made by plaster fixation of left hind limb in straight position for six weeks by modified Videman method.The model success criteria was evaluated by the Lequesne MG score of knee joint behavior≥4 points.Akupotomye group received akupotomye release treatment for“top of the cranium”,“upper of the external iliac”,and“lower of the internal iliac”,intervention once every seven days,a total of four interventions.The blank group and model group underwent the same grasping and fixation,but no akupotomye treatment.One week after the intervention,real-time tissue elastography was performed to measure the elastic strain rate ratio to evaluate the changes in tissue hardness of rectus femoris muscle.Masson staining was used to observe the changes of tissue morphology and collagen fiber content of rectus femoris muscle.Results Compared with the blank group,the elastic strain rate ratio and collagen volume fraction of rectus femoris muscle in the model group were all increased(P<0.05).Compared with the model group,the ratio of elastic strain rate and collagen volume fraction of rectus femoris in the akupotomye group decreased significantly(P<0.05).Conclusion Akupotomye intervention can improve the hardness of rectus femoris tissue in KOA rabbits and promote the restore of chronic injury fibrosis of rectus femoris.It can be one of the mechanisms of acupotomy in treating KOA.