趋化因子CXCL11及其受体CXCR7与系统性红斑狼疮疾病活动相关性研究

Study on the correlation between chemotactic factor CXCL11 and its receptor CXCR7 with disease activity in systemic lupus erythematosus

目的 探讨CXC型趋化因子(CXC)配体11及其受体CXCR7与系统性红斑狼疮(SLE)疾病活动的相关性。方法 选择40例SLE患者为江苏省徐州市中心医院(以下简称“我院”)风湿免疫科2018年10月至2020年10月初次发病患者作为SLE组。根据SLE疾病活动指数将其分为疾病无活动组(10例)、轻度疾病活动组(10例)、活动组(20例);另选我院同期20名健康体检者作为对照组。分别采用酶联免疫吸附试验、流式细胞术检测SLE组和健康对照组外周血中CXCL11、CD3^(+)CXCR7^(+)T细胞、CD19^(+)CXCR7^(+)B细胞表达情况,分析CXCL11、CXCR7与SLE疾病活动关系。结果 SLE组外周血中CD3^(+)CXCR7^(+)T细胞、CD19^(+)CXCR7^(+)B细胞及CXCL11表达水平高于健康对照组,差异有高度统计学意义(P <0.01)。不同疾病活动组SLE患者CD19^(+)CXCR7^(+)、CD3^(+)CXCR7^(+)、CXCL11水平比较,差异有统计学意义(P <0.05)。其中,疾病无活动组、轻度活动组、中重度活动组各指标高于健康对照组,轻度活动组、中重度活动组各指标高于疾病无活动组,中重度活动组各指标高于轻度活动组,差异有统计学意义(P <0.05)。SLE患者CD3^(+)CXCR7^(+)T细胞、CD19^(+)CXCR7^(+)B细胞、CXCL11表达与SLE疾病活动性指数评分、dsDNA、红细胞沉降率、C反应蛋白呈正相关(r> 0,P <0.05);与补体C3呈负相关(r <0,P <0.05)。结论 CXCL11、CXCR7在SLE患者体内不仅高表达,还可能参与SLE疾病发生发展和疾病活动。趋化因子CXCL11、CXCR7可作为判断SLE疾病活动指标。

Objective To investigate the correlation of CXC-type chemotactic factor(CXC) ligand 11 and its receptor CXCR7 with disease activity in systemic lupus erythematosus(SLE). Methods The enrolled 40 SLE patients were the first onset patients from October 2018 to October 2020 in the Department of Rheumatology and Immunology of Xuzhou Central Hospital of Jiangsu Province(hereinafter referred to as “our hospital”). According to the SLE disease activity index, they were divided into SLE non-disease activity group(10 cases), mild disease activity group(10 cases), moderate-severe disease activity group(20 cases);a total of 20 healthy subjects in our hospital during the same period were selected as the control group. Enzyme-linked immunosorbent assay and flow cytometry were used to detect the expressions of CXCL11, CD3^(+)CXCR7^(+)T cells, and CD19^(+)CXCR7^(+)B cells in peripheral blood of SLE patient group and healthy control group, respectively, while the relationship between CXCL11, CXCR7 and SLE disease activity were analyzed. Results The expression levels of CD3^(+)CXCR7^(+)T cells, CD19^(+)CXCR7^(+)B cells, and CXCL11 in peripheral blood of the SLE group were higher than those of the healthy control group, and the differences were highly statistically significant(P < 0.01). There were significant differences in the levels of CD19^(+)CXCR7^(+), CD3^(+)CXCR7^(+), and CXCL11 in SLE patients in different disease activity groups(P < 0.05). Among them, the indicators in the inactive disease group, the mild activity group, and the moderately severe activity group were higher than those in the healthy control group, while the indicators of the mild activity group and the moderate to severe activity group were higher than those of the inactive disease group, the indexes in the moderate-severe activity group were higher than those in the mild activity group, and the differences were statistically significant(P < 0.05). The expressions of CD3^(+)CXCR7^(+)T cells, CD19^(+)CXCR7^(+)B cells, and CXCL11 in SLE patients were positively correlated with systemic lupus erythematosus disease activity index score, dsDNA, erythrocyte sedimentation rate, and C-reactive protein(r > 0, P < 0.05);negatively correlated with complement C3(r < 0, P < 0.05).Conclusion CXCL11 and CXCR7 are not only highly expressed in SLE patients, but may also be involved in the occurrence, development and disease activity of SLE. Chemokines CXCL11 and CXCR7 can be used as indicators of SLE disease activity.